谷子和狗尾草WOX转录因子基因的全基因组鉴定与分析  

Genome-wide Identification and Analysis of WOX Transcription Factor Genes in Setaria italic and Setaria viridis

作　　者：李雪垠 武懿茂 张凤洁[2] LI Xueyin;WU Yimao;ZAHNG Fengjie(College of Life Sciences,Shanxi Agricultural University,Taigu 030801,China;College of Agronomy,Shanxi Agricultural University,Taigu 030801,China)

机构地区：[1]山西农业大学生命科学学院,山西太谷030801 [2]山西农业大学农学院,山西太谷030801

出　　处：《山西农业科学》2019年第6期957-962,共6页Journal of Shanxi Agricultural Sciences

基　　金：国家自然科学基金项目(31801434);山西省优秀博士来晋工作奖励资金科研项目(SXYBKY201725);山西农业大学科技创新基金项目(2017YJ28);山西省重点研发项目(201703D211008)

摘　　要：WOX转录因子主要功能涉及植物组织发育、器官形成和干细胞维持等。为了深入研究谷子和狗尾草WOX转录因子基因家族,为后续狗尾草属植物生长发育相关WOX基因的克隆和功能研究奠定理论基础,研究鉴定了谷子和狗尾草WOX转录因子家族基因,并进行了生物信息学分析。结果表明,谷子和狗尾草基因组分别包含13,12个WOX基因,谷子和狗尾草共有12对直系同源基因,这些同源基因具有相似的基因结构,谷子和狗尾草WOX基因内含子相位为0或1;谷子和狗尾草WOX基因蛋白质基序Motif1,Motif3,Motif4和Motif5较为保守;SiWOX1等基因启动子包含AACA_motif,CAT-box,GCN4_motif,RY-element等元件,说明它们可能参与调控胚乳或种子发育、或分生组织形成;SiWOX11和SvWOX10这2个基因在根组织中表达量非常高,说明这2个基因可能与根系发育密切相关。WOX transcription factors are mainly involved in plant tissue development,organ formation and stem cell maintenance,and so on.To further study the WOX transcription factor gene family of Setaria italic and Setaria viridis,and lay a theoretical foundation for cloning and functional study of WOX genes related to growth and development of Setaria plants,WOX transcription factor family genes of Setaria italica and Setaria viridis were identified and analyzed by bioinformatic methods in this study.The result showed that there were 13 and 12 WOX genes in the genomes of Setaria italica and Setaria viridis,respectively.There were 12 pairs of homologous genes in the two genomes.These homologous genes had similar gene structures.The intron phases of the WOX genes in Setaria italic and Setaria viridis were 0 or 1.Motif-1,Motif-3,Motif-4 and Motif-5 of the WOX proteins in Setaria italic and Setaria viridis were conservative.The promoters of some SiWOX genes,such as SiWOX1,included AACA_motif,CAT-box,GCN4_motif and RY-element,which indicated that these genes might be involved in regulating endosperm or seed development,or meristem formation.The expression of SiWOX11 and SvWOX10 was very high in root tissue,which indicated that they might be closely related to root development.

关 键 词：谷子 狗尾草 WOX 转录因子 生物信息学 基因表达 

分 类 号：S330[农业科学—作物遗传育种]