α-Glucosidase Inhibitory Activity-guided Identification of Compounds from Clerodendrum bungei Steud by HPLC-ESI-QTOF-MS/MS  

α-葡萄糖苷酶抑制剂活性指导下的HPLC-Q-TOF-MS/MS鉴定臭牡丹中化学成分（英文）

作　　者：HUANG Xiao-Long WAN Dan SHU Jun FANG Liang-Zi ZOU Du-Zhun ZHANG Shui-Han 黄小龙;万丹;舒骏;方良子;邹笃准;张水寒(湖南中医药大学,湖南长沙410208;湖南省中医药研究院中药研究所,湖南长沙410006)

机构地区：[1]Hunan University of Chinese Medicine,Changsha,Hunan 410208,China [2]Research Institute of Chinese Medicine,Hunan Academy of Chinese Medicine,Changsha,Hunan 410006,China

出　　处：《Digital Chinese Medicine》2019年第1期41-49,共9页数字中医药（英文）

基　　金：the funding support from the China National Natural Science Foundation Funding Project(NO.81503452);Natural Science Foundation of Hunan Province,China(No.14JJ4066)

摘　　要：Objective To identify the compounds withα-glucosidase inhibitory activity from Clerodendrum bungei Steud(Chou Mu Dan,臭牡丹)using HPLC-ESI-QTOF-MS/MS.Methods The ethanol extracts of Clerodendrum bungei Steud(Chou Mu Dan,臭牡丹)were partitioned with petroleum ether,ethyl acetate,n-butanol,and water.The assay forα-glucosidase inhibitory activity revealed strongα-glucosidase inhibitory activity in the ethyl acetate fraction,and the bioactive compounds present in this fraction were identified by the HPLCESI-QTOF-MS/MS method.Results A total of 29 compounds were determined,among the identified bioactive components;these included 12 phenylethanoid glycosides(compounds 5,6,17,20-22,24),7 flavonoids(compounds 10,19,23,25-28),5 phenolic acids(compounds 2-4,7,9),and 5 other compounds.Compounds 2-4,7,9-10,12-13,15,19,and 26,with a potentialα-glucosidase inhibitory activity,have been reported previously.Conclusions Our results show that the methodology used in this study is feasible,credible,and rapid in identifying known compounds and also for characterizing new natural glucosidase inhibitory candidates from Clerodendrum bungei Steud(Chou Mu Dan,臭牡丹).目的本研究旨在通过HPLC-Q-TOF-MS/MS方法确定臭牡丹中抑制α-葡萄糖苷酶的活性成分。方法臭牡丹乙醇提取物分别用石油醚、乙酸乙酯、正丁醇萃取得到3个提取部位;利用体外α-葡萄糖苷酶活性测定3个提取物的α-葡萄糖苷酶抑制活性,然后通过HPLC-ESI-QTOF-MS/MS方法鉴定乙酸乙酯部位的α-葡萄糖苷酶抑制活性成分。结果我们共鉴定出29个化合物,其中有12种苯乙醇苷(化合物5-6,12-17,20-22,24),7种黄酮类化物(化合物10,19,23,25-28)和5种酚酸(化合物2-4,7,9)以及5种其他化合物。通过查阅相关文献,初步确定化合物2-4,7,9-10,12-13,15,19和26具有潜在α-葡糖苷酶活抑制活性。结论本研究所应用的方法可行,可靠并且能快速地鉴定已知化合物,以及鉴定臭牡丹中新的天然α-葡萄糖苷酶抑制候选物。

关 键 词：α-glucosidase inhibitor Clerodendrum bungei Steud(Chou Mu Dan 臭牡丹) HPLC-ESI-QTOF-MS/MS 

分 类 号：R284.1[医药卫生—中药学] O657.63[医药卫生—中医学]