动物药羊胆水质量标准研究  

Quality Standard of Fel Capra Seu Ovis

作　　者：刘春花[1,2,3] 熊丹丹 潘洁[1,2] 刘绍欢 李勇军[1] 王爱民 王永林[1,4] UU Chunhua;XIONG Dandan;PAN Jie;UU Shaohuan;LI Yongjun;WANG Aimin;WANG Yonglin(Engineering Research Center for the Development and Application of Ethnic Medicine and TCM,Ministry of Education,State Key laboratory of Functions and Applications of Medicinal Plants,Guizhou Medical University,Guiyang,Guizhou,China 550004;Guizhou Provincial Key laboratory of Pharmaceutics,Guizhou Medical University,Guiyang,Guizhou,China 550004;School of Pharmacy,Guizhou Medical University,Guiyang,Guizhou,China 550004;Guizhou Provincial Engineering Research Center for the Development and Application of Ethnic Medicine and TCM,Guizhou Medical University,Guiyang,Guizhou,China 550004)

机构地区：[1]贵州医科大学民族药与中药开发应用教育部工程研究中心·省部共建药用植物功效与利用国家重点实验室,贵州贵阳550004 [2]贵州医科大学·贵州省药物制剂重点实验室,贵州贵阳550004 [3]贵州医科大学药学院,贵州贵阳550004 [4]贵州医科大学·贵州省民族药与中药开发应用工程技术研究中心,贵州贵阳550004

出　　处：《中国药业》2019年第11期26-29,共4页China Pharmaceuticals

基　　金：贵州省科技计划项目[黔科合平台人才〔2016〕5613,黔科合平台人才〔2016〕5677,黔科合基础〔2018〕1128,黔科合平台人才〔2017〕5718];贵州省国家级、省级创新创业训练计划项目[DC 201710660006];贵州省教育厅青年科技人才成长项目[黔教合KY字〔2017〕156]

摘　　要：目的建立羊胆水药材的质量标准。方法采用薄层色谱法对羊胆水进行定性鉴别。采用高效液相色谱(HPLC)法对羊胆水中胆酸含量进行测定,色谱柱为Phenomenex Luna-C18柱(250 mm×4.6 mm,5μm),流动相为乙腈-0.1%磷酸溶液(45∶55),流速为1.0 mL/min,检测波长为192 nm,进样量为5μL,柱温为35℃。结果羊胆水药材的薄层色谱斑点清晰,与对照药材羊胆粉主斑点一致;总固体含量范围0.0461~0.0760 g/mL;胆酸在0.4225~6.7600 g/L范围内与峰面积线性关系良好(r=0.9995);专属性、精密度、稳定性均良好;平均加样回收率为98.58%,RSD为1.43%(n=9);10批羊胆水药材中胆酸含量范围为7.537~19.660 g/L。结论该方法操作简单、准确、稳定、可靠,可为羊胆水的质量控制提供参考。Objective To establish the quality standard of Fel Capra seu ovis.Methods TLC method was adopted to identify Fel Capra seu oris.HPLC method was used for content determination of cholic acid in Fel Capra seu ovis,the chromatographic column was Phenomenex Luna-C(18)column(250 mm×4.6 mm,5μm),the mobile phase was acetonitrile-0.1%phosphoric acid solution(45∶55),the flow rate was 1.0 m L/min,the detection wavelength was 192 nm,the sample size was 5μL,and the column temperature was 35℃.Results The TLC spots of Fel Capra seu ovis were clear and consistent with the main spots of the reference crude herb.The content of total solids was in the range of 0.0461-0.0760 g/m L.The cholic acid showed good linear relationship in the range of 0.4225-6.7600 g/L(r=0.9995).The specificity,precision and stability were all good.The average recovery was 98.58%and RSD was 1.43%(n=9).The content of cholic acid in 10 batches of Fel Capra seu ovis in the range of 7.537-19.660 g/L.Conclusion The method is simple,accurate,stable and reliable,which can provide reference for the quality control of Fel Capra seu ovis.

关 键 词：羊胆水 薄层色谱法 总固体 胆酸 高效液相色谱法 质量控制 

分 类 号：R932[医药卫生—生药学] R282.74[医药卫生—药学]