不同产地姜黄药材高效液相色谱指纹图谱与化学模式识别研究  

HPLC Fingerprint and Chemical Pattern Recognition of Curcuma Longa from Different Regions

作　　者：黄卫娟[1] 韦卓纯 姜松 林绘 杨丽玲[1] HUANG Weijuan;WEI Zhuochun;JIANG Song;LIN Hui;YANG Liling(Department of Pharmacy,The Dongguan Affiliated Hospital of Medical College of Jinan University,Dongguan,Guangdong,China 523900)

机构地区：[1]暨南大学医学院附属东莞医院药学部

出　　处：《中国药业》2019年第12期24-27,共4页China Pharmaceuticals

基　　金：广东省中医药局面上科研项目[20171274]

摘　　要：目的建立姜黄药材的高效液相色谱(HPLC)指纹图谱,结合化学模式识别技术,为姜黄药材鉴别和质量评价提供依据。方法色谱柱为Shimadzu ODS-C18柱(150 mm×4. 6mm,5μm),流动相为乙腈-0. 2%甲酸溶液(梯度洗脱),流速为1. 0mL/min,柱温为35℃,检测波长为254 nm,进样量为10μL。采用“中药色谱指纹图谱相似度评价系统(2004 A版)”计算相似度,结合聚类分析和主成分分析对姜黄药材进行模式识别。结果姜黄药材的HPLC对照指纹图谱中有11个共有峰,并指认了其中3个共有峰成分;10批药材可聚为3类,分析确定了4个主成分。结论该HPLC指纹图谱结合化学模式识别可为姜黄药材的质量控制和品质评价提供参考。Objective To establish an HPLC fingerprint of Curcuma longa,and to provide the basis for the identification and quality evaluation of Curcuma longa by combining with chemical pattern recognition. Methods The chromatographic column was Shimadzu ODS-C18 column( 150 mm × 4. 6 mm,5 μm),the mobile phase was acetonitrile-0. 2% formic acid solution( gradient elution),the flow rate was 1. 0 m L/min,the column temperature was 35 ℃,the detection wavelength was 254 nm,and the sample size was 10 μL. The similarity was calculated by the Similarity Evaluation System for Chromatographic Fingerprint of TCM( 2004 A edition). The pattern recognition of Curcuma longa was carried out by cluster analysis and principal component analysis. Results There were 11 common peaks in the HPLC reference fingerprint of Curcuma longa and 3 common peaks were identified. 10 batches of medicinal materials could be grouped into 3 categories and 4 main components were identified. Conclusion The HPLC fingerprint of Curcuma longa combined with chemical pattern recognition can provide reference for quality control and quality evaluation of Curcuma longa.

关 键 词：姜黄 高效液相色谱指纹图谱 化学模式识别 主成分分析 聚类分析 

分 类 号：R932[医药卫生—生药学] R284.1[医药卫生—药学]