高效液相色谱法测定心可宁胶囊中4种皂苷含量  

Content Determination of Four Triterpene Saponins in Xinkening Capsules by HPLC

作　　者：赵袁丰 龚竹青 赵义雄 吴杰 ZHAO Yuanfeng;GONG Zhuqing;ZHAO Yixiong;WU Jie(Qiandongnan People's Hospital,Qiandongnan,Guizhou,China 556000;Guizhou Feiyunling Pharmaceutical Co.,Ltd.,Qiandongnan,Guizhou,China 556000)

机构地区：[1]贵州省黔东南苗族侗族自治州人民医院,贵州黔东南556000 [2]贵州飞云岭药业股份有限公司,贵州黔东南556000

出　　处：《中国药业》2019年第12期37-39,共3页China Pharmaceuticals

摘　　要：目的建立测定心可宁胶囊中三七皂苷R1、人参皂苷Rb1、人参皂苷Rg1、人参皂苷Re含量的高效液相色谱法。方法色谱柱为Diamonsil C18柱(250 mm×4. 6 mm,5μm),流动相为乙腈-水(梯度洗脱),流速为1. 0 m L/min,柱温为30℃,检测波长为203 nm,进样量为20μL。结果 4种皂苷成分的质量浓度分别在0. 020 8~0. 104 0 g/L(r=0. 999 9),0. 201 2~1. 006 0 g/L(r=0. 999 8),0. 180 2~0. 901 0 g/L(r=0. 999 9),0. 050 4~0. 252 0 g/L(r=0. 999 9)范围内与峰面积线性关系良好,平均回收率分别为99. 57%,100. 46%,100. 12%,100. 61%,RSD分别为1. 52%,1. 51%,1. 33%,1. 37%(n=9)。结论该方法简便,结果可靠,可用于测定心可宁胶囊中三七皂苷R1、人参皂苷Rb1、人参皂苷Rg1、人参皂苷Re的含量。Objective To establish an HPLC method for the content determine of notoginsenoside R1,ginsenoside Rb1,ginsenoside Rg1 and ginsenoside Re in Xinkening Capsules. Methods The chromatographic column was Diamonsil C18 column( 250 mm × 4. 6 mm,5 μm),the mobile phase was acetonitrile-water( gradient elution),the flow rate was 1. 0 m L/min,the column temperature was 30 ℃,the detection wavelength was 203 nm,and the sample size was 20 μL. Results Notoginsenoside R1,ginsenoside Rb1,ginsenoside Rg1 and ginsenoside Re showed good linear relationship in the ranges of 0. 020 8-0. 104 0 g/L( r = 0. 999 9),0. 201 2-1. 006 0 g/L( r = 0. 999 8),0. 180 2-0. 901 0 g/L( r = 0. 999 9),0. 050 4-0. 252 0 g/L( r = 0. 999 9),the average recovery rates were 99. 57%,100. 46%,100. 12% and100. 61%,RSDs were 1. 52%,1. 51%,1. 33% and 1. 37%( n = 9). Conclusion The method is simple and reliable,which can be used for the content determination of notoginsenoside R1,ginsenoside Rb1,ginsenoside Rg1 and ginsenoside Re in Xinkening Capsules.

关 键 词：高效液相色谱法 心可宁胶囊 三七皂苷R1 人参皂苷RB1 人参皂苷RG1 人参皂苷RE 含量测定 

分 类 号：R932[医药卫生—生药学] R284.1[医药卫生—药学]