左归丸对绝经后骨质疏松妇女Th17/Treg亚群偏移的影响  

Regulating effect of Zuoguiwan on Th17/Treg shift in postmenopausal osteoporosis

作　　者：谢宝华 赵霖 朱肖肖 尹训强 周宪宾[2] 李霞[2] XIE Baohua;ZHAO Lin;ZHU Xiaoxiao;YIN Xunqiang;ZHOU Xianbin;LI Xia(College of Basic Medicine,Jining Medical Univercity,Jining 272067,China;Shandong Academy of Medical Sciences,Jinan 250062,China)

机构地区：[1]济宁医学院基础医学院,济宁272067 [2]山东省医学科学院,济南250062

出　　处：《济宁医学院学报》2019年第3期166-170,共5页Journal of Jining Medical University

基　　金：国家自然科学基金项目(81373670,81704116);济宁医学院青年基金项目(JYQ14KJ29)

摘　　要：目的探讨左归丸对绝经后妇女骨密度和Th17/Treg亚群偏移的影响及其机制。方法选择2016年1月至2017年11月在山东中医药大学附属医院查体妇女共45例纳入未绝经组、绝经组和左归丸组各15例为研究对象,电化学发光法检测血清雌二醇(E2)水平,双能X线骨密度仪测定第2~4腰椎(L2-4)前后位骨密度(BMD),流式细胞术检测血清Th17/Treg亚群比例,Westernblot法检测血清Th17/Treg亚群特异性核转录因子(RORγt、Foxp3)蛋白表达水平,RT-PCR法检测血清Th17/Treg亚群特征性细胞因子(IL-17AmRNA、IL-10mRNA)表达水平。结果与未绝经组比较,绝经组血清E2水平及BMD均明显降低,Th17亚群比例明显升高,Treg亚群比例明显降低,差异具有统计学意义(P<0.05),T细胞向Th17亚群偏移;左归丸治疗后,与绝经组比较,左归丸组BMD明显升高,Th17亚群比例明显降低,Treg亚群比例明显升高,T细胞向Treg亚群偏移,RORγt蛋白表达明显降低,Foxp3蛋白表达明显升高,IL-17AmRNA表达降低,IL-10mRNA表达升高,差异具有统计学意义(P<0.05),血清E2表达无显著性差异(P>0.05);相关性分析表明,BMD与Th17亚群水平呈显著负相关(P<0.05),与Treg亚群水平呈显著正相关(P<0.05)。结论女性绝经后骨密度降低与Th17/Treg亚群向Th17偏移有密切关系,左归丸能调节T细胞亚群特异性核转录因子表达,诱导Treg亚群分化,逆转Th17/Treg亚群失衡状态,提高骨密度。Objective To investigate the effect and the mechanism of Zuoguiwan on bone mineral density(BMD)and shift of Th17/Treg of postmenopause women.Methods 45 cases of healthy examined women(15 cases of premenopause women,15 cases of postmenopause women and 15 cases of postmenopause women taking Zuoguiwan)in hospital were selected in this study.The serum estradiol(E 2)was assessed by electrothemiluminescence immunoassay.Bone mineral density(BMD)of lumbar vertebrae 2~4 was measured with dual energy X-ray absorptiometry.The percentages of Th17/Treg subsets were detected by flow cytometry.RORγt and Foxp3 were detected by Western blot.IL-17A mRNA and IL-10 mRNA were detected by reverse transcription polymerase chain reaction(RT-PCR).Results Compared with premenopause women,both E 2 and BMD of postmenopause women were decreased obviously.The percentage of Th17 subset was increased obviously,and the percentage of Treg subset were decreased obviously.The difference was statistically significant(P<0.05).T-cells subsets offset into Th17 subset.After Zuoguiwan administration,BMD of postmenopause women was increased obviously.The percentage of Th17 subset was decreased obviously,and the percentage of Treg was increased obviously.T-cells subsets offset into Treg subset.The expression of RORγt was decreased obviously,while the expression of Foxp3 was increased obviously.The expression of IL-17A mRNA was decreased obviously,while the expression of IL-10 mRNA was increased obviously.The difference was statistically signficant(P<0.05).There was no significant difference in the expression of serum E 2(P>0.05).BMD was positively correlated with the percentage of Treg subset(P<0.05)and was negatively correlated with the percentage of Th17 subset(P<0.05).Conclusion There was a close correlation between BMD and Th17/Treg shifts to Th17 after menopause.Zuoguiwan could improve BMD of postmenopause women via regulating the differentiation of Th17/Treg subgroups.

关 键 词：绝经后骨质疏松 TH17 TREG 左归丸 雌激素 

分 类 号：R259[医药卫生—中西医结合]