MiR-3880对奶山羊乳腺上皮细胞OGR1基因的靶向调控作用  

MiR-3880 Regulates the Expression of OGR1 Gene in Mammary Epithelial Cells of Dairy Goats

作　　者：侯金星[1] 安小鹏[2] 杜晓岩 曹斌云[2] 沈文正[1] 李云甫[1] HOU Jinxing;AN Xiaopeng;DU Xiaoyan;CAO Binyun;SHEN Wenzheng;LI Yunpu(Yangling Vocational Technical College,Yangling,Shaanxi 712100;College of Animal Science and Technology,Northwest A&F University,Yangling,Shaanxi 712100)

机构地区：[1]杨凌职业技术学院,陕西杨凌712100 [2]西北农林科技大学动物科技学院,陕西杨凌712100

出　　处：《家畜生态学报》2019年第5期13-17,共5页Journal of Domestic Animal Ecology

基　　金：陕西省自然科学基金(2018JM3006);杨凌职业技术学院科学研究基金(A2017003);国家自然科学基金青年项目(31601925)

摘　　要：为研究miR-3880对奶山羊OGR1基因的靶向调节作用,试验选用泌乳期奶山羊乳腺上皮细胞为研究材料,采用生物信息学和双荧光素酶报告载体试验,初步确定OGR1为miR-3880的靶基因。利用RT-qPCR和Westernblot方法进一步研究miR-3880对OGR1的靶向作用。结果显示,miR-3880通过与OGR1基因3ˊUTR结合调控其mRNA和蛋白的表达。本试验为研究miR-3880对乳腺上皮细胞泌乳功能的调节作用提供试验基础。In order to study the targeted regulation of miR-3880 on the OGR1 gene in dairy goats,the mammary epithelial cells of lactating dairy goats were selected as the research materials.The bioinformatics and dual luciferase reporter vector experiments were used to determine the OGR1 as the target gene of miR-3880.The targeting effect of miR-3880 on OGR1 was further investigated by RT-qPCR and western blot.The results showed that miR-3880 regulated the expression of mRNA and protein by binding to the OGR1 gene 3ˊUTR.This experiment provides a basis for studying the regulation of miR-3880 on the lactation function of mammary epithelial cells.

关 键 词：MiR-3880 OGR1 乳腺上皮细胞 奶山羊 

分 类 号：S811.5[农业科学—畜牧学]