藏绵羊eNOS基因外显子8多态位点检测及高原适应性分析  

The Polymorphisms of Tibet Sheep eNOS Gene exon8 and its Association with Adaptability of Plateau

作　　者：穷达 巴桑 唐晓惠 强巴央宗 张浩[2] QIONG Da;BA Sang;TANG Xiao-hui;Qiangba yangzong;ZHANG Hao(Animal Science College,Tibet Agriculture & Animal Husbandry University /Key Laboratory of Animal Breeding & Reproduction of Tibet Autonomous Region,Nyingchi Tibet,860000;College of Animal Science & Technology,China Agriculture University,Beijing 100094,China)

机构地区：[1]西藏农牧学院动物科学学院/西藏自治区动物遗传育种与繁殖重点实验室,西藏林芝860000 [2]中国农业大学动物科学技术学院,北京100193

出　　处：《高原农业》2019年第3期277-281,共5页Journal of Plateau Agriculture

基　　金：西藏自治区科技厅自然基金(厅校联合基金)项目(2016ZR-NY-01);西藏自治区教育厅高校青年教师创新支持计划(QCZ2016-52)

摘　　要：本研究旨在研究内皮型一氧化氮合酶(eNOS)外显子多态性与藏绵羊高原(高海拔低氧)适应的相关性。以藏绵羊、宁夏滩羊、内蒙古羊为研究对象,利用PCR测序法检测eNOS基因exon8序列多态位点和基因型。结果发现,绵羊eNOS基因exon8序列中存在2个SNPs位点,分别是exon8-A335C和exon8-G419A;其中exon8-A335C位点有3种基因型,高、低海拔绵羊群体间基因型分布差异显著,藏羊群体中A等位基因频率较高,可能对其高原适应具有调控作用。The association of Tibet sheep eNOS gene exon8 polymorphisms with adaptability of plateau was studied in this research. Tibetan sheep, Tan sheep and Mongolian sheep were selected to analyze the polymorphism of eNOS Gene exon8 by the direct sequencing method.The results showed that there were two mutation sites in sheep eNOS gene exon8, one of which might be related to altitude adaptation.

关 键 词：绵羊 ENOS基因 SNP 高原适应性 

分 类 号：S826[农业科学—畜牧学]