香辛方外敷对肺癌骨癌痛小鼠炎性介质和MCP-1的影响  

Effect of Xiangxin Prescription on Inflammatory Medium and MCP-1 in Mice with Bone Metastatic Cancer Pain

作　　者：何佩珊[1] 冯兴中[1] 姜敏[1] 潘国凤[1] 杨公博[2] 杨萌 齐慧 HE Pei-shan;FENG Xing-zhong;JIANG Min(Department of Traditional Chinese Medicine,Beijing Shijitan Hospital,Capital Medical University,Beijing (100038),China)

机构地区：[1]首都医科大学附属北京世纪坛医院中医科,北京100038 [2]中国中医科学院西苑医院创伤科,北京100091 [3]安泰康生物技术(北京)有限公司,北京102200

出　　处：《中国中西医结合外科杂志》2019年第3期262-266,共5页Chinese Journal of Surgery of Integrated Traditional and Western Medicine

基　　金：北京市中医药科技发展资金项目(QN2014-06);北京市科技计划课题(z181100001718150);北京市科技专项(Z141107001814061);北京市卫生系统高层次卫生技术人才培养计划(2014-3-046);北京市中西医结合肿瘤诊疗中心建设项目;首都医科大学附属北京世纪坛医院院青年基金项目(2014-Q16)

摘　　要：目的:应用活体荧光成像肺癌骨转移癌模型,探讨香辛方外敷缓解骨转移癌痛的机制。方法:通过股骨内注射表达绿色荧光蛋白的人肺腺癌A549细胞建立肺癌骨转移癌模型,随机分为中药外敷组、扶他林组、模型组,每组10只。中药外敷组香辛方0.5g/(kg·d)外敷,造模后7~21d 1次/d;扶他林组0.1g/(kg·d)外涂,造模后7~21d 1次/d;模型组生理盐水0.5mL/只外涂,1次/d;另设假手术组生理盐水0.5mL/只外涂,1次/d。造模21d后检测小鼠血脊髓单核细胞趋化蛋白-1(MCP-1)、白介素-1β(IL-1β)、肿瘤坏死因子α(TNFα)以及白介素-6(IL-6)的表达水平。结果:MCP-1表达模型组脊髓MCP-1表达水平[(1.88±0.18)mg/L]比假手术组[(1.21±0.19)mg/L]高,中药外敷组脊髓MCP-1表达水平[(1.51±0.09)mg/L]比模型组和扶他林组[(1.67±0.11)mg/L]降低(均P<0.05)。IL-1β水平扶他林组[(99.11±10.52)ng/L]和模型组[(104.46±11.07)ng/L]IL-1β水平比假手术组[(75.69±16.57)ng/L]高,中药外敷组IL-1β水平[(85.97±11.88)ng/L]比模型组和扶他林组低(均P<0.05)。TNFα水平中药外敷组[(447.91±44.67)ng/L]、扶他林组[(504.14±44.30)ng/L]和模型组[(534.84±48.89)ng/L]TNFα水平均比假手术组[(357.69±35.96)ng/L]升高,中药外敷组TNFα水平比模型组和扶他林组低(均P<0.05)。IL-6水平各组小鼠IL-6水平差异无统计学意义(P>0.05)。结论:香辛方可能通过减少脊髓MCP-1蛋白的表达,抑制中枢敏化;同时降低血IL-1β和TNFα水平。本方可能通过下调炎性介质表达,起到缓解骨转移癌痛的作用。Objective To explore the mechanism of Xiangxin Prescription on relieving bone metastatic cancer pain using in vivo fluorescence imaging model of lung cancer with bone metastasis.Methods Lung cancer bone metastatic pain model was built by injecting human lung adenocarcinoma A549 cells expressing green fluorescent proteins into the femurs of 30 nude mice.After successful modeling,the mice were randomly divided into external application of Chinese herb group,Votalin group and model group,n=10.Sham operation group was also established with other 10 mice.The external application of Chinese herb group was externally given Xiangxin Prescription,0.5 g/(kg·d);the Votalin group was locally applied with Votalin,with the dose of 0.1 g/(kg·d);the model group and Sham operation group were embrocated with normal saline(0.5 mL).The external application in each group was kept for at least 6 h,once daily.For each group,monocyte chemotactic protein-1(MCP-1),interleukin-lβ(IL-lβ),tumour necrosis factorα(TNFα)and interleukin-6(IL-6)were observed after the treatment.Results The level of MCP-1 in model group[(1.88±0.18)mg/L]was higher than that in Sham operation group[(1.21±0.19)mg/L](P<0.05).The level of MCP-1 in Chinese herb group[(1.51±0.09)mg/L]was less than that in model group(P<0.05).The levels of IL-1βin Votalin group[(99.11±10.52)ng/L]and model group[(104.46±11.07)ng/L]were higher than that in Sham operation group[(75.69±16.57)ng/L](P<0.05).The level of IL-1βin Chinese herb group[(85.97±11.88)ng/L]was less than those in Votalin group and model group(P<0.05).The level of TNFαin Chinese herb group[(447.91±44.67)ng/L]was less than those in Votalin group[(504.14±44.30)ng/L]and model group[(534.84±48.89)ng/L](P<0.05).The difference of IL-6 level in each group was not significant(P>0.05).Conclusion Xiangxin Prescription could inhibit the expression of MCP-1 and reduce the levels of IL-1βand TNFα,which could help to relieve the bone metastatic cancer pain.

关 键 词：芳香中药 骨转移癌痛 荧光成像 炎性介质 单核细胞趋化蛋白-1 

分 类 号：Q95-33[生物学—动物学]