锦鸡儿(Caragana sinica)芽增殖与植株再生  

Bud Proliferation and Plantlet Regeneration of Caragana Sinica

作　　者：李花花[1] 康红梅[1] 苏苌[1] 李珊[1] Li Huahua;Kang Hongmei;Su Chang;Li Shan(Institute of Biology,Gansu Academy of Science,Lanzhou 730000,China;Gansu Academy of Science,Lanzhou 730000,China)

机构地区：[1]甘肃省科学院生物研究所

出　　处：《甘肃科学学报》2019年第3期35-38,共4页Journal of Gansu Sciences

基　　金：甘肃省自然科学基金(1508RJZA056);甘肃省科学院青年科技创新基金(2016QN-06);甘肃省科学院应用研究与开发项目(2017JK-07)

摘　　要：以锦鸡儿(Caragana sinica)新生茎段为外植体,进行组织培养和快速繁殖。结果表明:芽诱导的最佳培养基为MS+15μmol/L6-BA+1μmol/LNAA;增殖培养最佳培养基为MS+5μmol/L6-BA+0.1μmol/LNAA+2μmol/LGA3;以1/2MS+10g/L蔗糖为培养基,增施0.15%CO2可达到较好的生根效果;锦鸡儿茎段芽诱导较容易,但增殖培养需添加适当浓度的GA3;增施CO2可提高试管苗生根率和存活率。The fresh stem segment of Caragana sinica is taken as the explant for tissue culture and rapid propagation.Results:The optimal culture medium for bud induction is MS+15μmol/L 6-BA+1μmol/L NAA.The optimal culture medium for proliferation is MS+5μmol/L 6-BA+0.1μmol/L NAA+2μmol/L GA 3.With sucrose of 1/2MS+10 g/L as culture medium,the increasing of 0.15%CO 2 may deliver a better rooting effect.The bud induction at the stem segment of Caragana sinica is relatively easy but the proliferation culture requires GA 3 of appropriate concentration.The increasing of CO 2 may improve the rooting rate and survival rate of test-tube plantlet.

关 键 词：锦鸡儿 芽增殖 微繁殖 

分 类 号：Q943.1[生物学—植物学]