偏肿革裥菌降解木质素关键基因挖掘  

Key Genes of Lignin Degradation by Lenzites gibbosa

作　　者：赵清泉 池玉杰[1] 张健[1] 冯连荣[1] Zhao Qingquan;Chi Yujie;Zhang Jian;Feng Lianrong(Northeast Forestry University,Harbin 150040,P.R.China)

机构地区：[1]东北林业大学

出　　处：《东北林业大学学报》2019年第6期79-83,共5页Journal of Northeast Forestry University

基　　金：中央高校基本科研业务费专项资金项目(2572017AA17);中央高校基本科研业务费科技平台持续发展专项资金项目(2572018CP05);2018外国文教专家聘请计划高校重点项目(T2018013)

摘　　要：通过对白腐菌偏肿革裥菌( Lenzites gibbosa )在木质和非木质环境下的转录组进行测序,从而预测和筛选出 L.gibbosa 与木材降解有关的基因。采用高通量测序技术对木屑和非木屑处理条件下的菌丝样本进行转录组测序。利用eggNOG、GO等数据库注释方法对转录本进行比较分析,预测和筛选出 L.gibbosa与木材降解有关的基因。L.gibbosa 转录组测序两组试验组分别设置3个生物学重复样本,共得到6个样本42.71 Gb Clean Data,各样品Clean Data平均达到7.11 Gb,各样品的Reads与参考基因组的比对效率在88.51~91.37%。差异表达分析得到差异表达基因1 120个,其中上调370个,下调750个。差异基因被注释到GO数据库的有493个,注释到eggNOG数据库的有857个。eggNOG分析表明,差异基因表达多聚集在翻译后修饰、蛋白质折叠、蛋白分子伴侣;碳水化合物的运输和代谢;能量产生和转化;次生代谢产物的生物合成、运输和分解代谢等功能分类下。GO分析表明,显著性富集与频率较高的生物过程是氧化还原过程、氧化还原酶活性和木质素代谢过程。 L.gibbosa 降解木材与木质素分解代谢过程(GO:0046274)、氧化还原过程(GO:0055114)和氧化还原酶活性(GO:0016491)等3个基因本体功能类目密切相关。根据基因功能注释的结果得到7个与白腐菌降解木质素相关的重要差异表达基因。The genes related to wood degradation of Lenzites gibbosa were predicted and screened by sequencing the transcriptome of L.gibbosa in both woody and non-woody environments.High-throughput sequencing technology was used to sequence transcriptomes of mycelial samples of L.gibbosa with and without wood treatments.All transcripts were annotated in eggnog,GO databases to predict and screen for genes associated with wood degradation.A total of 42.71 Gb clean data were obtained from the transcriptome sequencing of L.gibbosa.The average clean data of each sample reach 7.11 Gb.The mapped ratio between the reads and reference genomes of each sample ranged from 88.51% to 91.37%.The 1 120 differentially expressed genes (DEGs) were obtained,among which 370 were up-regulated and 750 down-regulated.There were 493 into GO database and 857 into eggNOG database.By eggNOG analysis,differential gene expression was concentrated in post-translational modification,protein folding,protein chaperones;carbohydrate transport and metabolism;energy production and transformation;secondary metabolite biosynthesis,transport and catabolism.By GO analysis,the significant enrichment and frequency of biological processes are redox processes,oxidoreductase activities and lignin metabolism processes.L.gibbosa degradation of wood is closely related to lignin catabolic process (GO:0046274),oxidation-reduction process (GO:0055114) and oxidoreductase activity (GO:0016491).From gene functional annotation results,seven important differentially expressed genes related to the degradation of lignin by white rot fungus were obtained.

关 键 词：偏肿革裥菌 白腐菌 转录组 木质素 降解 

分 类 号：S763.11[农业科学—森林保护学]