纳米铜粒子生物合成及其对染料脱色的效果  

Biosynthesis of Copper Nanoparticles and Its Effect on Dye Decolorization

作　　者：杨阔[1] 孙海琼[1] 汪春蕾[1] 赵敏[1] Yang Kuo;Sun Haiqiong;Wang Chunlei;Zhao Min(Northeast Forestry University,Harbin 150040,P.R.China)

机构地区：[1]东北林业大学

出　　处：《东北林业大学学报》2019年第6期84-87,93,共5页Journal of Northeast Forestry University

基　　金：国家自然科学基金项目(31470489)

摘　　要：以从土壤样品中筛选出的,经形态学、生理生化特性及16SrDNA序列同源性分析鉴定为地衣芽孢杆菌(Bacilluslicheniformis)的菌株HLS为材料,研究其合成纳米铜粒子能力及对染料的脱色效果。结果表明:菌株HLS能够利用Cu(NO3)2在菌体细胞内合成纳米铜粒子,透射电镜观察破胞沉淀样品中含有粒径10~20nm的正六边形晶体;X射线粉末衍射(XRD)图谱显示在43.5°、50.6°、74.3°和89.9°处有衍射峰,分别对应Cu的(111)、(200)、(220)和(311)晶面2θ角,主要晶型为Cu(111)。纳米铜粒子在无介体的情况下24h对结晶紫与刚果红的脱色率分别达到93.2%和94.3%。介体2,2,6,6,-四甲基哌啶氧化物(TEMPO)能显著提高纳米铜粒子对靛蓝胭脂红和结晶紫的脱色率,丁香醛(SA)能显著提高纳米铜粒子对活性亮蓝的脱色率。The HLS strain screened from soil samples and identified as Bacillus licheniformis by morphological,physiological and biochemical characteristics and 16S rDNA sequence homology analysis was used to study its ability to synthesize copper nanoparticles and decolorize dyes.The copper nanoparticles were synthesized in the cell of the strain HLS co-culture with Cu(NO 3) 2.The hexagonal crystals with diameter of 10-20 nm were observed by transmission electron microscopy.The X-ray powder diffraction patterns (XRD) showed that there were diffraction peaks at 43.5°,50.6°,74.3° and 89.9°,corresponding to crystal surface 2 θ angle of Cu(111),(200),(200) and (311),respectively,and the main crystal type was Cu(111).The decolorization rate of crystal violet and Congo red by copper nanoparticles were 93.2% and 94.3% in 24 h,respectively.Mediator 2,2,6,6-tetramethylpiperidine oxide (TEMPO) could significantly improve the decolorization rate of indigo carmine and crystal violet.Syringaldehyde (SA) could significantly improve the decolorization rate of reactive brilliant blue.

关 键 词：纳米铜粒子 染料脱色 地衣芽孢杆菌 介体 

分 类 号：S936[农业科学—渔业资源]