中麻黄不同生长期3种药效成分的变化规律  

Change rules of three active constituents in Ephedra intermedia at different growth stages

作　　者：马晓辉[1,2] 晋玲 朱田田[1,2] 何微微 黄得栋[1,2] MA Xiao-hui;JIN Ling;ZHU Tian-tian;HE Wei-wei;HUANG De-dong(Gansu University of Chinese Medicine,Lanzhou 730000,China;Research Institute of Chinese ( Tibetan) Medicinal Resources,Gansu Universityof Chinese Medicine,Lanzhou 730000,China;Key Laboratory of Chemistry and Quality for TCM and Tibetan Medicine in Universities of Gansu Province,Lanzhou 730000,China)

机构地区：[1]甘肃中医药大学,甘肃兰州730000 [2]甘肃中医药大学中(藏)药资源研究所,甘肃兰州730000 [3]甘肃省高校中(藏)药化学质量研究省级重点实验室,甘肃兰州730000

出　　处：《中成药》2019年第6期1333-1338,共6页Chinese Traditional Patent Medicine

基　　金：中央财政引导地方科技创新平台项目(2016);科技基础性工作专项子课题(2013FY114500);2014高校基本科研业务费专项课题(甘财教[2014]63号06);甘肃省基础研究创新群体项目(1606RJIA323)

摘　　要：目的探讨中麻黄不同生长期3种药效成分(麻黄碱、伪麻黄碱、甲基麻黄碱)的变化规律。方法中麻黄1.44%磷酸提取物的分析采用Hypersil C18柱(250 mm×4.6 mm,10μm);流动相甲醇-0.092%磷酸溶液,梯度洗脱;体积流量1.0 mL/min;柱温25℃;检测波长210 nm。结果麻黄碱、伪麻黄碱、甲基麻黄碱分别在16.2~519μg/mL(r=0.9998)、14.9~447μg/mL(r=0.9999)、0.5~16μg/mL(r=0.9999)范围内线性关系良好,平均加样回收率95%~195%,RSD<3.0%。结论该方法准确稳定,重复性好,可用于中麻黄的质量控制。AIM To explore the change rules of three active constituents in Ephedra intermedia Schrenk ex Mey. at different growth stages.METHODS The analysis of 1.44% phosphoric acid extract of E. intermedia was performed on a 25 ℃ thermostatic Hypersil C18column(250 mm×4.6 mm,10 μm), with the mobile phase comprising of methanol-0.092% phosphoric acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength was set at 210 nm.RESULTS Three constituents showed good linear relationships within the ranges of 16.2-519 μg/mL(r=0.9998)、14.9-447 μg/mL(r=0.9999)、0.5-16 μg/mL(r=0.9999),whose average recoveries were 95%-195%,RSD<3.0%.CONCLUSION This accurate,stable and reproducible method can be used for the quality control of E. intermedia.

关 键 词：中麻黄 麻黄碱 伪麻黄碱 甲基麻黄碱 HPLC 

分 类 号：R284.1[医药卫生—中药学]