UPLC法测定不同海拔及不同采收期乌梅中枸橼酸和苹果酸  

Determination of citric acid and malic acid in Prunus mume from different altitudes and different gathering periods by UPLC

作　　者：群培 翟雪 金乾 杨正明 刘圆[4] QUN Pei;ZHAI Xue;JIN Qian;YANG Zheng-ming;LIU Yuan(College of Pharmacy,Southwest Minzu University,Chengdu 610041,China;Agriculture Bureau of Yuechi County,Guang’an City,Guang’an638300,China;College of Agronomy,Sichuan Agricultural University,Chengdu 611130,China;Ethnic Medicine Institute,Southwest MinzuUniversity,Chengdu 610041,China)

机构地区：[1]西南民族大学药学院,四川成都610041 [2]四川省广安市岳池县农业局,四川广安638300 [3]四川农业大学农学院,四川成都611130 [4]西南民族大学民族医药研究院,四川成都610041

出　　处：《中成药》2019年第6期1338-1343,共6页Chinese Traditional Patent Medicine

基　　金：国家科技支撑计划(2015BAC05B02);四川省科技支撑计划(2015FZ0026);西南民族大学中央高校基本科研业务费专项资金研究类项目(2018NZD10)

摘　　要：目的建立UPLC法测定不同海拔和不同采收期乌梅Prunus mume(Sieb.) Sieb. et Zucc.中枸橼酸和苹果酸的含有量。方法乌梅80%甲醇提取物的分析采用ACQUITY HSS C18色谱柱(2.1 mm×100 mm,1.8μm);流动相乙腈-0.2%磷酸水,梯度洗脱;体积流量0.2 mL/min;检测波长210 nm;柱温35℃。结果枸橼酸、苹果酸分别在0.516~1.596、0.496~1.627μg/mL范围内线性关系良好(R2≥0.9996);平均加样回收率分别为100.36%、100.39%,RSD 1.43%、1.54%。结论该方法准确稳定,重复性好,可用于乌梅的质量控制。AIM To establish a UPLC method for the content determination of citric acid and malic acid in Prunus mume(Sieb.) Sieb. et Zucc. from different altitudes and different gathering periods.METHODS The analysis of 80% methanol extract of P. mume was performed on a 35 ℃ thermostatic ACQUITY HSS C18column(2.1 mm×100 mm,1.8 μm), with the mobile phase comprising of acetonitrile-0.2% phosphoric acid flowing at 0.2 mL/min in a gradient elution manner,and the detection wavelength was set at 210 nm.RESULTS Citric acid and malic acid showed good linear relationships within their own ranges(R2≥0.9996), whose average recoveries were 100.36%、100.39%,with the RSDs of 1.43%、1.54%,respectively.CONCLUSION This accurate,stable and reproducible method can be used for the quality control of P. mume.

关 键 词：乌梅 枸橼酸 苹果酸 海拔 采收期 UPLC 

分 类 号：R284.1[医药卫生—中药学]