恩诺沙星对马氏珠母贝肠道微生物群落的影响  

Effects of enrofloxacin on intestinal microbial community of Pinctada fucata martensii

作　　者：洪嘉炜 陈明强[2,3] 邓正华 李有宁[2,3] 马振华 顾志峰[1] 王雨 HONG Jia-wei;CHEN Ming-qiang;DENG Zheng-hua;LI You-ning;MAZhen-hua;GU Zhi-feng;WANG Yu(Ocean College,Hainan University,Haikou 570228,China;Tropical Aquatic Research and Development Center, South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Sanya,Haikou 572018, China;Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization,Ministry of Agriculture and Rural Affairs,Guangzhou 510300,China)

机构地区：[1]海南大学海洋学院,海口570228 [2]中国水产科学研究院南海水产研究所热带水产研究开发中心,海南三亚572018 [3]农业农村部南海种质资源利用重点实验室,广州510300

出　　处：《南方农业学报》2019年第5期1104-1110,共7页Journal of Southern Agriculture

基　　金：海南省重点研发计划项目(ZDYF2017037,ZDYF2016086);广东省渔港建设和渔业产业发展专项(B201601-Z02);广西北海市科学研究与技术开发计划项目(20177038)

摘　　要：[目的]明确恩诺沙星对马氏珠母贝(Pinctada fucata martensii)肠道微生物群落的影响,了解其变化规律,为制定马氏珠母贝细菌感染的最佳治疗方案提供参考依据。[方法]采用16S rDNA测序定量评价不同恩诺沙星处理浓度(0、5和10 mg/L)下马氏珠母贝肠道微生物群落结构的变化情况,并运用生物信息学手段在门和属水平上比较分析各处理间马氏珠母贝肠道微生物群落结构的相似性和差异性。[结果]从9个马氏珠母贝肠道内容物样品(PA1、PA2、PA3、PB1、PB2、PB3、PC1、PC2和PC3)中共获得755159个有效序列,分别归属于9个门48个属。在门水平上,对照处理(PA,0 mg/L)马氏珠母贝肠道的优势菌门为变形菌门(Proteobacteria)、软壁菌门(Tenericutes)和厚壁菌门(Fir-micutes),相对丰度分别为40.65%、33.87%和15.75%;两个恩诺沙星处理(PB,5 mg/L;PC,10 mg/L)的优势菌均为变形菌门,相对丰度分别为91.61%和85.00%。在属水平上,PA处理马氏珠母贝肠道的优势菌属为支原体属(Mycoplas-ma)、微小杆菌属(Exiguobacterium)、柠檬酸细菌属(Citrobacter)和埃希氏菌志贺氏菌属(Escherichia-Shigella),相对丰度分别为26.33%、12.22%、10.62%和9.66%;PB处理的优势菌属为弧菌属(Vibrio),相对丰度为52.10%;PC处理的优势菌属为弧菌属和发光杆菌属(Photobacterium),相对丰度分别为40.24%和23.43%。操作分类单元(OTU)聚类分析结果表明,3个处理(PA、PB和PC)的共有OUTs为138个,占OTUs总数的54.76%;PB和PC处理的共有OUTs达176个,即这两个处理间具有更高的相似性。[结论]恩诺沙星处理浓度达5 mg/L时即对马氏珠母贝肠道微生物群落产生影响,并使其暴露于弧菌等致病菌的威胁下,因此在马氏珠母贝养殖生产中须谨慎使用恩诺沙星。【Objective】The aim of the study was to investigate the possible impact of enrofloxacin on Pinctada fucata martensii,find out the changing rule and provide a reference for the optimal treatment of bacterial infection in P. fucata martensii.【Method】The microbial shifts in the intestine of P. fucata martensii in response to enrofloxacin treatments at different dosages(0,5,and 10 mg/L)were quantitatively evaluated using 16S rDNA gene sequencing,and the similarities and differences of intestinal microbial community structure among different treatments were compared and analyzed by bioinformatics at the phylum and genus levels.【Result】A total of 755159 effective sequences were obtained from 9 intestinal contents samples of P. fucata martensii(PA1,PA2,PA3,PB1,PB2,PB3,PC1,PC2 and PC3),belonging to 48 genera and 9 phyla. At the phylum level,the dominant genera of the control(PA,0 mg/L)was Proteobacteria,Tenericutes and Firmicutes,whose relative abundances were 40.65%,33.87% and 15.75%,respectively. Nevertheless,the dominant phylum in two enrofloxacin treatment groups(PB,5 mg/L;PC,10 mg/L)were Proteobacteria,the relative abundance of which have reached 91.61% and 85.00% respectively. At the genus level,the dominant phylum of PA treatment were Mycoplasma, Exiguobacterium,Citrobacter,Escherichia-Shigella,the relative abundance of which were 26.33%,12.22%, 10.62% and 9.66%,respectively. Vibrio held the dominant position of PB,and its relative abundance was 52.10%. Vibrio and Photobacterium made up the most share of microbial community of PC,which collectively accounted for 40.24% and 23.43% respectively. OTU cluster analysis showed that there were 138 OTUs in three treatments(PA,PB and PC), accounting for 54.76% of the total OTUs. 176 OUTs were shared by PB and PC,which meaned that there was a higher similarity between the two treatments.【Conclusion】Overall,enrofloxacin alters the microbial community diversity of P. fucata martensii as the dosage reaches 5 mg/L,and exposes them to the threat of pathogenic bact

关 键 词：马氏珠母贝 恩诺沙星 肠道微生物 Α多样性 相对丰度 高通量测序 

分 类 号：S968.316[农业科学—水产养殖]