南方红豆杉野生种与栽培品种‘金锡杉’针叶代谢物的比较分析  

Comparison of Metabolites in the Needles of Taxus wallichiana var. mairei and Taxus wallichiana var. mairei cv. ‘Jinxishan’

作　　者：陈一鸣 王涛[2] 张凤姣 庄维兵[2] 束晓春[2] 王忠[2] 杨清[1] CHEN Yiming;WANG Tao;ZHANG Fengjiao;ZHUANG Weibing;SHU Xiaochun;WANG Zhong;YANG Qing(College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China;Institute of Botany, Jiangsu Province and Chinese Academy of Sciences (Nanjing Botanical Garden Mem. Sun Yat-Sen), Nanjing 210014, China)

机构地区：[1]南京农业大学生命科学学院,南京210095 [2]江苏省中国科学院植物研究所(南京中山植物园),南京210014

出　　处：《西北植物学报》2019年第5期801-807,共7页Acta Botanica Boreali-Occidentalia Sinica

基　　金：江苏省植物资源研究与利用重点实验室开放基金(JSPKLB201605,JSPKLB201806);中央财政林业科技推广示范项目(苏[2016]TG01)

摘　　要：利用广泛靶向代谢组的方法对相同生境下南方红豆杉野生种(Taxuswallichiana var.mairei)及栽培品种‘金锡杉’(Taxus wallichiana var.maireicv.‘Jinxishan’)针叶中代谢物含量差异进行分析。结果表明:(1)在野生种和栽培品种的针叶提取物中,共鉴定出689种代谢物并获得其相应的积分定量值,包括初生代谢物326种、次生代谢物334种和其他类成分29种。(2)定量分析结果显示,有71种代谢物在两种红豆杉中的表达差异显著,这些差异代谢物主要富集在糖代谢、脂质合成等初生代谢途径,以及黄酮类合成等次生代谢途径中。(3)在‘金锡杉’针叶中,大多数氨基酸(5种)和黄酮类代谢物(10种)含量远高于野生种,而糖代谢(3种)、脂类合成(4种)及TCA循环(3种)途径中的差异代谢物含量均远低于野生种。研究认为‘金锡杉’针叶中黄酮类次生代谢物含量升高归因于苯丙氨酸和酪氨酸代谢途径的协同调控,从而增强‘金锡杉’对外界环境的适应性;而‘金锡杉’中低含量的,涉及糖代谢、脂类生物合成以及TCA循环等途径的代谢物造成的能量供应不足,则通过合成大量的氨基酸类物质来维持平衡。Widely targeted metabolomics was used to study the difference of metabolite contents in the needles of Taxus wallichiana var . mairei and T. wallichiana var . mairei cv.‘Jinxishan’. The results showed that:(1) a total of 689 metabolites and their integral quantitative values were found, including 326 primary metabolites, 334 secondary metabolites and 29 other components.(2) The results of quantitative analysis showed 71 metabolite contents were significantly difference. Most of differential metabolites between T. wallichiana var . mairei and T. wallichiana var . mairei cv.‘Jinxishan’ were significant enriched in primary metabolic pathways, such as glycometabolism and lipid biosynthesis pathways, as well as secondary metabolic pathways such as flavonoids biosynthesis pathways.(3) A large number of metabolites in the amino acid and flavonoid biosynthesis pathways were significantly accumulated in T. wallichiana var. mairei cv.‘Jinxishan’, while the differential metabolites in sugar metabolism, lipid synthesis and TCA cycle pathways were mainly accumulated in T. wallichiana var. mairei . The study considered that the increase of flavonoid content was attributed to the co-regulation of phenylalanine and tyrosine metabolic pathways, which improved the adaptability of T. wallichiana var. mairei cv.‘Jinxishan’ to the environment. At the same time, the energy was supplied by synthesizing large amounts of amino acids to make up for the lack of energy, caused by the decrease of metabolite contents in glycometabolism, lipid biosynt hesis and TCA cycle pathways.

关 键 词：代谢组学 南方红豆杉 协同调控 

分 类 号：Q946[生物学—植物学] Q599