水杨酸盐诱发大鼠听皮层中TNF-α和GluA1表达的改变  

Expression of TNF-α and GluA1 in the Auditory Cortex in Salicylate-Exposed Rats

作　　者：李雅兰 李婷 王采集 葛佳丽 肖倩文 左健 赵泽祺 雷冠雄 张世丽 乔月华 LI Yalan;LI Ting;WANG Caiji;GE Jiali;XIAO Qianwen;ZUOJian;ZHAO Zeqi;LEI Guanxiong;ZHANG Shili;QIAO Yuehua(Xuzhou Medical University, Jiangsu 221004, China;Center for Morphology Research, Xuzhou Medical University, Jiangsu 221004, China;Jiangsu Provincial Laboratory of Artificial Auditory Engineering, Jiangsu 221004, China;Xuzhou Medical University Hospital, Jiangsu 221002, China;Xiangnan University, Hunan 423000, China)

机构地区：[1]徐州医科大学,江苏221004 [2]徐州医科大学形态学研究中心,江苏221004 [3]江苏省人工听觉工程实验室,江苏221004 [4]徐州医科大学附属医院,江苏221002 [5]湘南学院,湖南423000

出　　处：《中华耳科学杂志》2019年第3期382-387,共6页Chinese Journal of Otology

基　　金：国家自然科学基金(81470684);江苏省高等学校自然科学研究重大项目(18KJA320011);徐州市科技计划项目(重点研发项目KC17198)~~

摘　　要：目的通过检测水杨酸盐诱导耳鸣大鼠听皮层中TNF-α和AMPA受体亚基(GluA1)蛋白的表达,研究耳鸣大鼠听皮层是否出现炎症因子和膜受体表达的改变,探讨TNF-α在耳鸣中的作用机制。方法 24只SD大鼠,随机等分成4组:对照组、慢性注射7天组、慢性注射14天组、停药后恢复14天组。前脉冲抑制实验评估动物是否发生耳鸣样行为。Western Blot检测听皮层中TNF-α和GluA1蛋白表达。结果⑴长期注射水杨酸盐后,大鼠的前脉冲抑制率增加,差异有统计学意义(P<0.05)。⑵在慢性注射7天组,TNF-α和GluA1蛋白表达水平较对照组升高,差异有统计学意义(P=0.036,P=0.019,P<0.05);在慢性注射14天组,TNF-α和GluA1蛋白表达水平较对照组、停药后恢复14天组升高,差异有统计学意义(P=0.003,P=0.002,P<0.01)。⑶TNF-α和GluA1蛋白表达具有显著正相关(P=0.000,P<0.001)。结论耳鸣模型大鼠听皮层中TNF-α和GluA1蛋白表达均上调且两者变化有显著正相关性,推测TNF-α可能通过调节GluA1的表达导致大鼠耳鸣。Objective To investigate the central mechanisms of tinnitus in a rat model by examining expression levels of TNF-α and AMPA receptor subunit(GluA1) in the auditory cortex following salicylate exposure. Methods Twenty-four SD rats were randomly divided into a control group, a treatment group with daily salicylate injection for 7 days(S7), a treatment group with daily salicylate injection for 14 days(S14), and a group examined 14 days after salicylate administration(S14+R14). Salicylate-induced tinnitus-like behavior was assessed using the pre-pulse inhibition test.Western Blot was used to examine the expression of TNF-α and GluA1 in the auditory cortex. Results(1) After salicylate injection, the rate of pre-pulse inhibition increased(P<0.05).(2) Compared to the control group, the protein expression levels of TNF-α and GluA1 increased in the S7 treatment groups(P<0.05). Compared with the control and S14+R14 groups, the protein expression levels of TNF-α and GluA1 in S14 treatment group were significantly higher(P<0.01).(3) The protein expression levels of TNF-α and GluA1 were positively correlated(P<0.001). Conclusion The change of protein expressions levels of TNF-α and GluA1 in the auditory cortex and their correlation may be suggestive of a TNF-α modulated GluA1 elevation responsible for salicylate-induced tinnitus.

关 键 词：水杨酸盐 耳鸣TNF-α AMPA受体 听皮层 

分 类 号：R764[医药卫生—耳鼻咽喉科]