改良石蜡切片法观察金针菇子实体不同发育时期组织结构  

Tissue Structure Observation of Flammulina Filiformis Fruiting Bodies at Different Developmental Stages by Improved Paraffin Method

作　　者：王瑞娟[1] 万佳宁[1] 龚明[1] 茅文俊[1] 汪莹 唐利华[1] 尚俊军 杨慧[1] 鲍大鹏[1] WANG Ruijuan;WAN Jianing;GONG Ming;MAO Wenjun;WANG Ying;TANG Lihua;SHANG Junjun;YANG Hui;BAO Dapeng(Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences,Key Laboratory of Edible Fungi Resources and Utilization(South),Ministry of Agriculture,P.R.China,National Engineering Research Center of Edible Fungi,National R&D Center for Edible Fungi Processing,Key Laboratory of Agricultural Genetics and Breeding of Shanghai,Shanghai 201403 China)

机构地区：[1]上海市农业科学院食用菌研究所农业部南方食用菌资源利用重点实验室国家食用菌工程技术研究中心国家食用菌加工技术研发中心上海市农业遗传育种重点开放实验室

出　　处：《食用菌学报》2019年第2期54-58,I0008,I0009,共7页Acta Edulis Fungi

基　　金：上海市农业科学院卓越团队建设计划;上海自然科学基金(18ZR1416800);上海市食用菌产业技术体系建设专项资金[沪农科产字(2019)第9号];2016年度上海市级农口系统青年人才成长计划项目[沪农青字(2016)第1-27号]

摘　　要：运用改良石蜡切片法制备了金针菇(Flammulina filiformis)子实体不同发育时期的切片,观察其菌褶和菌柄的细胞结构。结果表明,以柠檬烯为透明剂,金针菇子实体组织结构清晰、完整、无皱缩。观察不同发育时期的金针菇子实体组织切片发现,金针菇原基内部有呈菌丝无规则扭结状态的菌盖结构,随着子实体不断发育,菌盖下端有菌褶结构分化,随后菌柄结构随菌盖发育而进一步分化,菌柄组织细胞状纵向紧密排列,横切面有维管束状细胞呈外紧密内疏松状排列且其间有孔洞出现,菌褶菌髓部呈菌丝与球泡状细胞共同存在的异型分布结构。The improved paraffin method was used to prepare sections of Flammulina filiformis fruiting bodies at different developmental stages, and to observe cell structure of gills and stipes. The results showed that F. filiformis fruiting body section was clear, intact and wrinkle-free with limonene as clearing agent. Observation of tissue sections of F. filiformis fruiting bodies showed that there was a pileus structure with irregularly twisted mycelium in primordium. With the development of fruiting body, the gills differentiated in the bottom of pileus;after that, the stipe structure was further differentiated with the development of pileus. The stipe cells were longitudinally closely aligned in F. filiformis. Viewing from the cross section, vascular bundle cells were arranged as compacting inside and loosening outside with cavities in it. A heteromorphic structure was observed, where mycelia and bulliform cells coexisted in gill trama.

关 键 词：柠檬烯 透明剂 子实体组织结构 

分 类 号：S646.15[农业科学—蔬菜学]