水鬼蕉生物碱对人肝癌HepG-2细胞凋亡的影响  

Effects of Alkaloids of Hymenocallis littoralis on Apoptosis of Human Hepatocellular Carcinoma HepG-2 Cells

作　　者：陈宁[1] 赵丹[1] 郑威[1] 李均 杜娟[1] 凌娜[1] 宋冬雪[1] 徐昶儒[1] CHEN Ning;ZHAO Dan;ZHENG Wei;LI Jun;DU Juan;LING Na;SONG Dong-xue;XU Chang-ru(Life Sciences and Environmental Sciences Research Center,Harbin 150076,China)

机构地区：[1]哈尔滨商业大学生命科学与环境科学研究中心

出　　处：《黑龙江农业科学》2019年第6期128-132,共5页Heilongjiang Agricultural Sciences

基　　金：黑龙江省博士后基金(LBH-Z16095);黑龙江省自然基金(H2017 001);哈尔滨市应用技术研究与开发项目(2016RQQXJ127)

摘　　要：为揭示水鬼蕉生物碱(AHL)对HepG-2细胞作用,采用FITC-Annexin V/PI双染色法检测AHL对HepG-2细胞凋亡的影响,并检测了AHL对HepG-2细胞内Ca2+浓度、活性氧(ROS)、pH的影响。结果表明:AHL可以通过细胞内ROS、Ca^2+、pH三者间相互作用而诱导细胞凋亡。In order to reveal the effect of alkaloids of Amaryllidaceae plant Hymenocallis littoralis (AHL) on HepG-2 cells, FITC-Annexin V/PI double staining was used to detect the effect of AHL on apoptosis of HepG-2 cells and on Ca^2+ concentration, reactive oxygen species (ROS) and pH in HepG-2 cells. The results show that: AHL could induce apoptosis through the interaction of ROS, Ca^2+, and pH in cells.

关 键 词：水鬼蕉生物碱 FITC-Annexin V/PI双染色法 CA^2+浓度 活性氧 pH 

分 类 号：R285[医药卫生—中药学]