半乳糖化卡培他滨前体脂质体包封率的测定  

Determination of Entrapment Efficiency of Galactoside Capecitabine Precursor Liposomes

作　　者：李季 Li Ji(The Third Affiliated Hospital of Jinzhou Medical University,Jinzhou 121000 China)

机构地区：[1]锦州医科大学附属第三医院

出　　处：《锦州医科大学学报》2019年第3期9-11,I0004,共4页Journal of Jinzhou Medical University

摘　　要：目的建立半乳糖化卡培他滨前体脂质体包封率的测定方法。方法采用阳离子交换树脂微柱离心法分离脂质体和游离药物,HPCE法测定卡培他滨浓度并计算包封率。采用未涂渍标准熔融石英毛细管柱,运行电压25kV,毛细管柱温25℃,检测波长240nm,压力进样5kPa×3s,电泳缓冲液为40mmol/L磷酸二氢钠(pH=3.2)溶液。结果测得3批制品包封率分别为72.5%、69.9%和71.2%。结论阳离子交换树脂-HPCE法检测半乳糖化卡培他滨前体脂质体包封率高效、经济、简便。Objective To establish a method for the determination of entrapment efficiency of galactoside capecitabine precursor liposomes. Methods Liposomes and free drugs were separated by cation exchange resin microcolumn centrifugation. The capecitabine concentration was determined by HPCE and entrapment efficiency was calculated. Uncoated standard fused silica capillary column was adopted. The operating voltage was 25 kV,capillary column temperature was 25 ℃,detection wavelength was 240 nm,pressure injection was 5 kPa ×3 s,and electrophoretic buffer was 40 mmol/L sodium dihydrogen phosphate (pH 3.2) solution. Results The Encapsulation efficiency of the 3 batches was 72.5%,69.9% and 71.2% respectively. Conclusion Cation exchange resin-HPCE method is efficient,economical and simple for the determination of encapsulation efficiency of galactosed capecitabine precursor liposomes.

关 键 词：卡培他滨 前体脂质体 阳离子交换树脂 HPCE 包封率 

分 类 号：R927[医药卫生—药学]