乳腺癌中SyK、HGF表达的相关性及预后的关系  

Correlation between SyK and HGF in Breast Cancer and Their Prognosis Significance

作　　者：庄晓苹 林琼琼 季剑乐 朱暐 Zhuang Xiaoping;Lin Qiongqiong;Ji Jianle(Department of Pathology,Wenzhou Hospital of Integrated Traditional Chinese and Western Medicine,Zhejiang 325000,China)

机构地区：[1]温州市中西医结合医院

出　　处：《医学研究杂志》2019年第5期126-129,共4页Journal of Medical Research

基　　金：浙江省温州市科技局计划项目(Y20140225)

摘　　要：目的探讨乳腺癌中脾酪氨酸激酶基因(spleen tyrosine kinase,SyK)、肝细胞生长因子(hepatocyte growth factor,HGF)表达的相关性及其与乳腺癌患者预后的关系。方法采用实时PCR方法分别检测120例乳腺癌组织、100例正常乳腺组织、100例纤维腺瘤组织之间的SyK和HGF基因表达,分析乳腺癌组织中二者的表达水平与临床病理学特征的关系,并对其表达的相关性以及与乳腺癌患者预后的关系进行了统计分析。结果SyK、HGF mRNA的表达在乳腺癌组织和正常乳腺组织及纤维腺瘤组织之间比较,差异有统计学意义(P<0.01);而二者表达在其他两组之间比较,差异无统计学意义(P>0.05)。SyK、HGF mRNA表达与乳腺癌的临床分期、脉管侵犯相关(P<0.05),但与年龄、肿块直径无关(P>0.05)。120例乳腺癌患者的随访结果为术后5年内死亡人数为8例,占6.67%(8/120),生存中位数时间为47个月,采用Kaplan-Meier法对120例乳腺癌患者分为两组,即比较组与预后组,结果显示对两组生存曲线整体比较的Log Rank检验结果为P=0.009,Breslow检验结果为P=0.018。按照Log Rank检验的结果,可以认为两组表达对患者的生存率有差异,预后组与生存状态呈负相关(r=-0.202,P<0.05)。结论SyK、HGF参与乳腺癌侵袭和转移中的作用,SyK缺失或HGF过表达提示患者预后不良,可作为乳腺癌预后判断的指标。Objective To investigate the correlation between SyK and HGF in breast cancer and their prognosis significance.Methods The expressions of SyK and HGF gene were detected by RT-PCR method in 120 samples of breast cancer,100 samples of normal breast tissue and 100 samples of fibroadenoma.We analyzed its relationship with clinicopathological parameters of breast cancer.The correlation between SyK and HGF and their prognosis significance were investigated. Results There were statistically significant differences between the expression levels of SyK and HGF of breast cancer,normal breast tissue and fibroadenoma (P<0.01).There were no statistically significant differences between the expression levels of SyK and HGF of normal breast tissue and fibroadenoma (P>0.05).These data showed that the expression of SyK and HGF is remarkably related to the clinincal stage and vascular invasion(P<0.05),but not to the age and tumor size.Follow-up results for 120 cases of breast cancer patients were 8 death cases withinfive years,accounted for 6.67%(8/120).And the median survival time was 47 months.With the method of Kaplan Meier,we divided these 120 breast cancer patients into two groups:comparison group and prognosis group.The results showed a comparison of two groups of overall survival curves of the Log Rank test results for P=0.009.And Breslow test results for P=0.018.According to the results of the Log Rank test,the prognosis group was negatively correlated with the survival status (r=-0.202,P<0.05).Conclusion SyK and HGF were involved in breast cancer invasion and metastasis.The deficiency of SyK and the high expression of HGF in breast cancer may be served as an indicator of the prognosis of breast cancer.

关 键 词：乳腺癌 脾酪氨酸激酶 肝细胞生长因子 反转录-聚合酶链反应 

分 类 号：R75[医药卫生—皮肤病学与性病学]