模拟舰船冲击运动对坐姿状态下兔腰椎间盘损伤效应的研究  

A study on the injury of lumber intervertebral discs after simulated ship shock motion to the rabbits in sitting position

作　　者：陈佳海 阮狄克[1] 胡明 徐成 刘立洋 王静 巨圆圆 李军 CHEN Jiahai;RUAN Dike;HU Ming;XU Cheng;LIU Liyang;WANG Jing;JU Yuanyuan;LI Jun(Department of Orthopedic Surgery,the Sixth Medical Center,Chinese PLA General Hospital,Beijing 100048,China;NO.910 Hospital of PLA, Quanzhou Fujian, 362000, China)

机构地区：[1]中国人民解放军总医院第六医学中心骨科,北京100048 [2]中国人民解放军第910医院,福建泉州362000

出　　处：《转化医学杂志》2019年第3期175-178,共4页Translational Medicine Journal

基　　金：军队后勤科研计划指令项目(BHJ14L011)

摘　　要：目的 观察模拟舰船冲击运动对坐姿状态下兔腰椎间盘的损伤效应。方法 实验动物随机分为对照组(冲击速度0m/s)、轻度冲击组(冲击速度2.5m/s)、重度冲击组(冲击速度4.8m/s),将兔置于模拟冲击平台上,以不同的冲击速度对坐姿状态下的兔进行冲击,于冲击后3h行X线检查;于冲击后第1周、第4周及第12周时对兔腰椎间盘行磁共振成像(magneticresonanceimaging,MRI)检查,比较不同组的实验动物不同时刻腰椎间盘的Pfirrmann分级;对腰椎间盘行苏木精-伊红(Hematoxylin-eosin,HE)染色、天狼星红染色,并于镜下观察兔腰椎间盘损伤后的变化。结果 实验中无即刻死亡的动物,对照组与轻度冲击组、重度冲击组兔的腰椎间盘Pfirrmann分级比较差异有统计学意义(P<0.05);轻度与重度冲击组兔的腰椎间盘Pfirrmann分级比较差异无统计学意义(P>0.05),退变的腰椎间盘在HE染色下可见髓核细胞形态退变,纤维细胞增生,纤维结构紊乱。在天狼星红染色下可见纤维成分增多、紊乱,髓核区域缩小。结论 模拟舰船冲击运动对坐姿状态下兔的腰椎间盘可造成不同程度的损伤,在冲击伤后12周内发生了退变。Objective To explore the effect of the injury on lumber intervertebral discs of the rabbits in sitting postures under ship shock injury. Methods The experiment rabbits were divided into 3 groups,the control group( velocity 0 m/s),the mild impact group( velocity 2. 5 m/s),and the severe impact group( velocity 4. 8 m/s). Simulating the ship shock motion to the sitting rabbits in different speed,X ray examination at 3 hours after ship shock motion while MRI examination at 1 week,4 weeks and 12 weeks after ship shock motion. Pfirrmann grading of lumber intervertebral disc was compared in different groups at different time. Hematoxylin-eosin( HE) staining and SirusRed staining were done to observe the injury of intervertebral disc. Results No rabbit died immediately after the simulating ship shock motion. There was significant difference in the Pfirrmann grading of lumber intervertebral disc in the control group when compared with that in the mild impact group and the severe impact group( P<0. 05). There was no statistical difference in lumber intervertebral disc grading between the mild and severe shock groups( P>0. 05). The HE staining of the degenerative discs showed the morphology of the nucleus pulpous cell changed,fibrocyte increased and fiber structure was disorganized. The Sirus-Red staining of the degenerative discs showed the fiber composition increased,disordered,and the nucleus area reduced. Conclusion The results showed that ship shock motion damaged the lumber interbertebral discs of the rabbits in the sitting position and degenerative change of the lumber intervertebral discs happened in the following 12 weeks.

关 键 词：舰船冲击运动 兔 坐姿 腰椎间盘损伤 

分 类 号：R681.5[医药卫生—骨科学] R83[医药卫生—外科学]