快速纠正乙二胺四乙酸依赖性假性血小板减少的临床新方法  

A new clinical method for rapid correction of EDTA-dependent pseudothrombocytopenia

作　　者：高晓玲[1] 吴惠玲[1] 朱江贤[1] Gao Xiaoling;Wu Huiling(Zhu Jiangxian The People's Hospital of Yuxi City,Yunnan Province 653100)

机构地区：[1]云南省玉溪市人民医院

出　　处：《中国社区医师》2019年第16期104-105,共2页Chinese Community Doctors

摘　　要：目的:建立一种新的准确、简便纠正乙二胺四乙酸(EDTA)依赖性假性血小板减少(EDTA-PTCP)的处理方法。方法:收治EDTA-PTCP患者118例。采用含乙二胺四乙酸二钾(EDTA-K2)干粉的抗凝管二次抗凝处理标本,同时取患者末梢血手工法计数血小板,比较两种方法处理结果。结果:118例样本中,116例用上述方法处理后,检测结果与末梢血手工法计数结果比较,差异无统计学意义(P>0.05);涂片、染色镜检观察血小板无集聚,呈散在分布,能纠正EDTA-PTCP;2例样本用上述方法处理后,未能纠正EDTA-PTCP,涂片、染色观察血小板仍集聚。结论:临床工作中发现EDTA-PTCP时,可采用含EDTA-K2干粉的抗凝管纠正,该方法避免了二次采血,能缩短患者标本检测平均时间,提高了检验效率。Objective:To establish a rapid,correct and available detection method for EDTA-dependent pseudothrombocytopenia. Methods:118 patients with EDTA-PTCP were selected,the anticoagulation tube containing the EDTA-K2 dry powder was used for secondary anticoagulation treatment,the peripheral blood was manually counted to count the platelets,and the results of the two methods were compared.Results:Of the 18 samples,116 were treated with the above method.There was no significant difference between the results of the test and those of the manual counting of peripheral blood(P>0.05),no aggregation of platelets was observed by smear and staining microscopy,which showed scattered distribution and could correct EDTA-dependent pseudothrombocytopenia.Two cases of EDTA-dependent pseudothrombocytopenia were not corrected after treatment with the above methods, and platelet aggregation was observed by smear and staining.Conclusion:When EDTA-PTCP happend in daily work,using the microcentrifuge tube with EDTA-K2 dry powder to correct the PLT result can avoid second blood drawing,shorten the testing time,improve the test efficiency.

关 键 词：血小板假性减少 抗凝剂 检测方法 

分 类 号：R446.11[医药卫生—诊断学]