他汀类药物对食管癌细胞增殖及Akt蛋白表达影响  

EFFECT OF STATINS ON ESOPHAGEAL CANCER CELL PROLIFERATION AND PROTEIN KINASE B EXPRESSION

作　　者：侯广杰[1] 何苡[1] 杨光煜[1] HOU Guangjie;HE Yi;YANG Guangyu(Department of Thoracic Surgery, Henan Province People’s Hospital, Zhengzhou 450003, China)

机构地区：[1]河南省人民医院胸外科

出　　处：《青岛大学学报（医学版）》2019年第4期434-436,441,共4页Journal of Qingdao University(Medical Sciences)

基　　金：河南省科技攻关计划项目(122102310618)

摘　　要：目的探讨他汀类药物对食管癌细胞增殖及蛋白激酶B(Akt)蛋白表达影响。方法取食管鳞癌(ESCC)细胞株进行培养,以不同时间(24、48h)和不同浓度(15、45μmol/L)的普伐他汀、辛伐他汀进行干预(4个实验组),并设立空白对照组。分别检测各组细胞增殖和丙二醛(MDA)含量,以及辛伐他汀对细胞周期和细胞凋亡、AktmRNA表达的影响。结果普伐他汀、15μmol/L辛伐他汀干预癌细胞时,对细胞增殖均无明显影响(P>0.05),45μmol/L辛伐他汀能够显著抑制癌细胞增殖(F=5.345、16.383,P<0.05)。各浓度普伐他汀干预癌细胞时,MDA含量均无明显变化(P>0.05);各浓度辛伐他汀干预癌细胞时,MDA含量均有明显上升(F=5.913、7.435,P<0.05)。辛伐他汀干预癌细胞时,G0/G1期细胞实验组明显高于对照组,S期、G2/M期细胞实验组明显低于对照组(t=2.975~10.027,P<0.05)。各浓度辛伐他汀干预癌细胞时,AktmRNA表达均明显下降(F=6.541、7.548,P<0.05)。结论辛伐他汀对食管癌细胞确有抑制细胞增殖、促进细胞凋亡的作用,并能够抑制Akt蛋白的表达。Objective To investigate the effect of statins on the proliferation of esophageal cancer cells and the expression of protein kinase B (Akt). Methods An esophageal squamous cell carcinoma (ESCC) cell line was cultured, and the cells were divided into 4 experimental groups and were treated with pravastatin or simvastatin at different concentrations (15 and 45 μmol/L). A blank control group was also set up. Cell proliferation and malondialdehyde (MDA) level were measured for all groups, and the effect of simvastatin on cell cycle, cell apoptosis, and mRNA expression of Akt was analyzed. Results Pravastatin and 15 μmol/L simvastatin had no significant effect on the proliferation of ESCC cells ( P >0.05), and 45 μmol/L simvastatin significantly in- hibited the proliferation of cancer cells ( F =5.345 and 16.383, P <0.05). The cancer cells treated with different concentrations of pravastatin had no significant change in MDA content ( P >0.05), while those treated with different concentrations of simvastatin had a significant increase in MDA content ( F =5.913 and 7.435, P <0.05). When the cancer cells were treated with simvastatin, compared with the control group, the experimental group had a significantly higher number of cells in the G 0 /G 1 phase and a signi- ficantly lower number of cells in the S phase or G 2 /M phase ( t =2.975-10.027, P <0.05). The cancer cells treated with different concentrations of simvastatin had a significant reduction in the mRNA expression of Akt ( F =6.541 and 7.548, P <0.05). Conclusion Simvastatin can inhibit the proliferation and promote the apoptosis of esophageal cancer cells and inhibit the expression of Akt protein.

关 键 词：食管肿瘤 普伐他丁 细胞增殖 细胞凋亡 癌基因蛋白质v-Akt 

分 类 号：R735.1[医药卫生—肿瘤] R972.6[医药卫生—临床医学]