脂联素对慢性间歇性缺氧所致大鼠胰岛细胞线粒体损伤的作用  

Adiponectin ameliorated pancreatic islet mitochondrial injury induced by chronic intermittent hypoxia

作　　者：丁文筱 张蔷[1] 葛路遥 董艳彬 丁宁 张希龙 Ding Wenxiao;Zhang Qiang;Ge Luyao;Dong Yanbin;Ding Ning;Zhang Xilong(Department of Respirology,Zhongda Hospital,School of Medicine,Southeast University,Nanjing 210009,China;Department of Respirology and Critical Care Medicine,the First Affiliated Hospital of Nanjing Medical University,Nanjing 210029,China)

机构地区：[1]东南大学附属中大医院呼吸内科,南京210009 [2]南京医科大学第一附属医院呼吸与危重症医学科,南京210029

出　　处：《中华医学杂志》2019年第18期1380-1384,共5页National Medical Journal of China

基　　金：国家自然科学基金(81700090,81770086);东南大学-南京医科大学合作研究项目(2242017K3DN44).

摘　　要：目的探讨慢性间歇性缺氧(CIH)致大鼠胰岛细胞损伤及脂联素的保护机制。方法36只雄性成年SD大鼠采用随机数字表法分为正常对照(NC)组、CIH组及CIH+脂联素组。CIH及CIH+脂联素组大鼠接受间歇缺氧处理,CIH+脂联素组大鼠同时接受脂联素处理。实验每天进行8h,共35d。实验第0、7、14、21、28、35天检测空腹血糖及空腹胰岛素水平。造模后检测血清脂联素、胰岛细胞三磷酸腺苷(ATP)含量、超氧化物歧化酶(SOD)活性、丙二醛含量、线粒体氧化磷酸化功能及线粒体合成相关基因mRNA表达水平、线粒体及胞质细胞色素C蛋白表达水平。结果三组大鼠各时间点空腹血糖及空腹胰岛素差异均无统计学意义(均P>0.05)。与NC及CIH+脂联素组相比,CIH组血清脂联素水平[(7 265±2 209)ng/ml、(6 536±1 678)ng/ml比(4 923±1 742)ng/ml,均P<0.05]、胰岛细胞ATP含量[(30.92±1.12)nmol/mg、(26.55±0.72)nmol/mg比(20.22±1.47)nmol/mg,均P<0.05]、SOD活性、线粒体/胞质细胞色素C蛋白表达水平比值、线粒体氧化磷酸化功能及合成相关基因mRNA表达水平均降低,丙二醛含量升高(均P<0.05)。与NC组相比,CIH+脂联素组血清脂联素水平差异无统计学意义,丙二醛含量升高,余指标均降低(均P<0.05)。结论脂联素通过抑制氧化应激减轻CIH所致胰岛细胞线粒体损伤。Objective To explore the effect of adiponectin (APN) on islet injury induced by chronic intermittent hypoxia (CIH).Methods Thirty-six SD rats were randomly divided into three groups: Normal control (NC),CIH,and CIH + APN groups.The rats in the CIH and CIH+APN groups received an intermittent hypoxia exposure while the rats in NC group received the room air only.The rats in CIH+APN group received the intravenous injection of APN.The intermittent hypoxia events persisted 8 hours a day and last for 35 days.The fasting blood glucose and fasting insulin were detected at the time of 0,7,14,21,28,and 35 day.After 35 days,the level of serum adiponectin,and adenosine triphosphate (ATP) level,superoxide dismutase (SOD),malondialdehyde (MDA),the mRNA levels of mitochondrial oxidative phosphorylation function and mitochondrial synthesis gene,and the protein level of mitochondrial and cytoplasmic cytochrome C of pancreatic islet were detected.Results The glucose and insulin level had no statistically differences among three groups at different time points (all P>0.05).However,compared with NC and CIH+APN groups,CIH reduced the serum adiponectin [(7 265±2 209) ng/ml,(6 536±1 678) ng/ml vs (4 923±1 742) ng/ml,both P<0.05],ATP levels [(30.92±1.12) nmol/mg,(26.55±0.72) nmol/mg vs (20.22±1.47) nmol/mg,both P<0.05],mRNA levels of mitochondria oxidative phosphorylation function and mitochondrial synthesis gene,the activity of SOD,and the rate of mitochondrial/cytoplasmic cytochrome C protein level while increased the MDA level in pancreatic islet.Compared with NC group,the MDA level increased (P<0.05) and the APN level had no statistically difference,while the level of other indicators decreased in CIH+APN group (all P<0.05).Conclusion APN ameliorates the pancreatic islet injury induced by CIH through inhibition of oxidative stress.

关 键 词：睡眠呼吸暂停 阻塞性 脂联素 线粒体 氧化性应激 慢性间歇性缺氧 

分 类 号：R766[医药卫生—耳鼻咽喉科] R587.1[医药卫生—临床医学] R-332