人参花蕾中的酰化黄酮醇苷类化合物及其α-葡萄糖苷酶抑制活性  

Acylated kaempferol glycosides from flower buds of Panax ginseng and theirα-glucosidase inhibitory activity

作　　者：李珂珂 弓晓杰 LI Ke-ke;GONG Xiao-jie(Department of Biological Engineering,Life Science College of Dalian Minzu University,Dalian 116600,China)

机构地区：[1]大连民族大学生命科学学院生物工程系,辽宁大连116600

出　　处：《中草药》2019年第10期2285-2289,共5页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(81603272);大连市高层次人才创新支持计划(2017RQ152);辽宁省高等学校创新团队支持计划(LT2016017);大连市科技创新基金(2018J12SN062);大连市第二批领军人才资助项目

摘　　要：目的研究人参花蕾中的黄酮类化学成分及其抑制α-葡萄糖苷酶的活性。方法采用MCI gel、硅胶柱色谱及半制备高效液相色谱等方法进行分离、纯化,NMR、MS等波谱数据进行结构鉴定。运用96微孔板测定化合物的体外抑制α-葡萄糖苷酶活性。结果从人参花蕾醇提物的醋酸乙酯层中分离得到了5个黄酮类化合物,分别鉴定为山柰酚3-O-(2″,3″-二-反式-对-香豆酰基)-α-L-鼠李糖苷(1)、山柰酚3-O-(3″,4″-二-反式-对-香豆酰基)-α-L-鼠李糖苷(2)、山柰酚3-O-(3″-顺式-对-香豆酰基,4″-反式-对-香豆酰基)-α-L-鼠李糖苷(3)、山柰酚3-O-(2″,4″-二-反式-对-香豆酰基)-α-L-鼠李糖苷(4)及山柰酚3-O-(2″,4″-二-顺式-对-香豆酰基)-α-L-鼠李糖苷(5)。α-葡萄糖苷酶体外抑制活性测定结果表明,化合物3对α-葡萄糖苷酶有较强抑制作用。结论化合物1～5均为首次从人参属中分离得到,人参花蕾中的苯丙酰基酰化黄酮醇苷类成分对α-葡萄糖苷酶有一定的抑制作用。Objective To study the chemical constituents of flavonoids and its α-glucosidase inhibitory activity from the flower buds of Panax ginseng. Methods The compounds were isolated and purified by MCI gel, silica gel and semi-preparative HPLC chromatography, and the structures were elucidated based on the NMR and MS data. The α-glucosidase inhibitory activities of the isolated compounds in vitro were determined by 96-well microtiter plate. Results From ethyl acetate fraction of alcohol extract of P.ginseng flower buds, five flavonoids were isolated and identified as kaempferol-3-O-(2″,3″-di-E-p-coumaroyl)-α-L-rhamnoside(1),kaempferol-3-O-(3″,4″-di-E-p-coumaroyl)-α-L-rhamnoside(2), kaempferol-3-O-(3″-Z-p-coumaroyl,4″-E-p-coumaroyl)-α-L-rhamnoside(3), kaempferol-3-O-(2″,4″-di-E-p-coumaroyl)-α-L-rhamnoside(4), and kaempferol-3-O-(2″,4″-di-Z-p-coumaroyl)-α-L-rhamnoside(5). The inhibitory activity of α-glucosidase in vitro showed that compound 3 had stronger inhibitory effect on α-glucosidase.Conclusion Compounds 1-5 are isolated from this genus for the first time, and the phenylpropionyl acylated flavonol glycosides in P. ginseng flower buds have some inhibitory effect on α-glucosidase in vitro.

关 键 词：人参属 人参花蕾 酰化黄酮醇苷 苯丙酰基酰化黄酮醇苷 α-葡萄糖苷酶抑制活性 

分 类 号：R284.1[医药卫生—中药学]