雷公藤多苷对溃疡性结肠炎模型大鼠炎症相关因子及核因子-κB、β-arrestin1、Bcl-2表达的影响  

Effect of Tripterygium Glycosides on Inflammation-related Factors and Expression of Nuclear Factor-κB,β-arrestin1 and Bcl-2 in Rats with Ulcerative Colitis

作　　者：艾燕 吴昌旭 AI Yan;WU Changxu(General Hospital of Pingmei Shenma Medical Group, Pingdingshan Henan China 467000)

机构地区：[1]平煤神马医疗集团总医院

出　　处：《中医学报》2019年第5期963-966,共4页Acta Chinese Medicine

基　　金：国家自然科学基金项目(81201012,81371438)

摘　　要：目的:观察雷公藤多苷对溃疡性结肠炎模型大鼠肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、转化生长因子-β1(transforming growth factor-β1,TGF-β1)、核因子-κB(nuclear factor-κB,NF-κB)、β-arrestin1、Bcl-2表达的影响。方法:60只雄性Wistar大鼠随机分为对照组、模型组、雷公藤多苷组和硫唑嘌呤组。除对照组外,其他组分别采用TNBS/乙醇联合灌肠的方法制备溃疡性结肠炎模型。各组分别给予相应药物连续灌胃,对照组和模型组给予生理盐水灌胃14 d,第16天处死大鼠,取结肠标本,分别选择Elisa和Western blot法检测结肠组织中TNF-α、TGF-β1及NF-κB、β-arrestin1、Bcl-2蛋白的表达。结果:造模3 d后模型组大鼠体质量降低、精神倦怠、懒动,出现不同程度的腹泻及便血,疾病活动指数评分高于对照组(P<0.05),大鼠结肠肠管变粗、肠壁与周围组织粘连,黏膜多处片状充血、水肿。与对照组比较,模型组的大鼠结肠组织TGF-β1明显降低(P<0.05),TNF-α及NF-κB、β-arrestin1、Bcl-2表达水平明显升高(P<0.05);与模型组比较,雷公藤多苷组与硫唑嘌呤组TGF-β1显著增高(P<0.05),TNF-α及NF-κB、β-arrestin1、Bcl-2蛋白表达明显下降(P<0.05)。结论:雷公藤多苷对溃疡性结肠炎模型大鼠有治疗作用,其机制可能与升高TGF-β1,降低炎症因子水平和NF-κB、β-arrestin1、Bcl-2蛋白表达有关。Objective:To observe the effects of tripterygium glycosides on the expression of tumor necrosis factor-α(TNF-α),transforming growth factor-β1(TGF-β1) and nuclear factor-κB(NF-κB),β-arrestin1 and Bcl-2 in rats with ulcerative colitis.Methods:60 male Wistar rats were randomly divided into control group,model group,tripterygium polyglycoside group and azathioprine group.Except the control group,other groups used TNBS/ethanol combined enema to prepare ulcerative colitis rat model.Each group was given corresponding drugs by continuous gavage.The control group and model group were given saline for 14 days,and the rats were executed on the 16 th day.Colon specimens were taken.The expression of TNF-α,TGF-β1,NF-κB,β-arrestin1 and Bcl-2 protein in colon tissue were detected by Elisa and Western Blotting methods,respectively.Results:After 3 days of modeling,the rats in the model group had lower body mass,mental fatigue,laziness,diarrhea and hematochezia in varying degrees.The DAI score was higher than that in the control group(P<0.01).The colon intestinal canal became thicker,the intestinal wall adhered to the surrounding tissues,and the mucosa became flaky congestion and edema in many places.Compared with the control group,TGF-β1 in the colon tissue of the model group was significantly lower(P<0.01).The expression levels of TNF-α,NF-κB,β-arrestin1 and Bcl-2 increased significantly(P<0.01).Compared with the model group,the expression of TGFβ1 was significantly increased in tripterygium glycoside group and azathioprine group(P<0.01),while the expression of TNF-α,NF-κB,β-arrestin1 and Bcl-2 was significantly decreased(P<0.01,P<0.05).Conclusion:Tripterygium glycosides can treat ulcerative colitis in rats,and its mechanism may be related to elevated TGFβ1,decreased inflammatory factors and NF-κB,β-arrestin1 and Bcl-2 protein expression.

关 键 词：溃疡性结肠炎 雷公藤多苷片 炎症相关因子 β-arrestin1 NF-κB 大鼠 

分 类 号：R285.5[医药卫生—中药学]