百合花青素苷呈色类型中3种R2R3-MYBs基因的差异表达  

Differential Expression of Three R2R3-MYBs Genes Regulating Anthocyanin Pigmentation Patterns in Lilium spp.

作　　者：曹雨薇[1] 徐雷锋[1] 杨盼盼[1] 徐华 何国仁 唐玉超 任君芳 明军[1] CAO Yuwei;XU Leifeng;YANG Panpan;XU Hua;HE Guoren;TANG Yuchao;REN Junfang;MING Jun(The Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China;Aba Prefecture Institute of Forestry Science and Technology,Wenchuan,Sichuan 623000,China)

机构地区：[1]中国农业科学院蔬菜花卉研究所,北京100081 [2]阿坝州林业科学技术研究所,四川汶川623000

出　　处：《园艺学报》2019年第5期955-963,共9页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(31672196;31801899);国家重点基础研究专项(2018YFD1000405);国家社会公益研究专项(2005DIB3J022);中国农业科学院创新工程项目;农业部园艺作物生物学与种质创制重点实验室项目

摘　　要：以亚洲百合系、LA系(麝香百合×亚洲百合)以及野生原种等7个不同呈色类型百合花被片为试验材料,通过RT-PCR扩增检测3种R2R3-MYBs基因Lh MYB12、LhMYB6和LrMYB15在花被片斑点和斑线中的表达,筛选出具有调控作用的LhMYB12和Lh MYB6,并对其进行全长序列的克隆和序列分析。结果表明:LhMYB12在亚洲百合花被片的斑线和溅泼状斑点中表达,LhMYB12和LhMYB6在LA系百合斑线和斑点中表达,在野生原种南川百合(Lilium rosthornii)、湖北百合(L. henryi)和欧洲百合(L. martagon)的花被片不同呈色类型中只有LhMYB12表达,未检测到LrMYB15参与本试验材料的调控。获得的LhMYB12和Lh MYB6全长序列分别为763和797bp,包括ORF区744和705bp,编码247个和234个氨基酸,与已经克隆的百合属LhMYB12、Lh SorMYB12和LhMYB6相似性为78%～88%。表明LhMYB12在百合杂交种和野生原种的呈色类型中均起重要作用,是一个广泛存在的R2R3-MYBs。LhMYB6在LA中与LhMYB12共同发挥作用。本研究揭示了调控百合花被片花青素苷不同呈色类型的R2R3-MYBs在不同品种类群中的差异表达。Anthocyanin accumulation in different parts of tepal developed various pigmentation patterns,but the expression patterns of R2R3-MYBs regulating various anthocyanin pigmentation patterns in Lilium spp.are not well understood.In this study,expression patterns of LhMYB12,LhMYB6 and LrMYB15 genes regulating various anthocyanin pigmentation patterns were performed in seven different pigmentation patterns of materials[Asiatic hybrids,LA(Lilium longiflorum×Asiatic hybrids)and wild species].LhMYB12 and LhMYB6,cloned full-length cDNA and analyzed gene sequences,were considered to be regulated gene related to anthocyanin pigmentation patterns.The study showed LhMYB12 was expressed in linear spots at edge of tepals and splatter spots in Asiatic hybrids.But LhMYB12 and LhMYB6 were expressed in linear spots at edge of tepals and splatter spots in LA hybrids.And only LhMYB12 was expressed in anthocyanin pigmentation patterns of wild species(L.rosthornii,L.henryi and L.martagon).However,anthocyanin pigmentation patterns of these parameters were not observed in LrMYB15.The full-length of LhMYB12 and LhMYB6 was 763 and 797 bp containing open reading frame of 744 and 705 bp,which encoded 247 and 234 amino acids respectively.They were homologous sequence with published LhMYB12,LhSorMYB12 and LhMYB6,sharing 78%to 88%similarity with them.The results indicated that LhMYB12 played an important role in the regulation of anthocyanin pigmentation patterns in hybrids and wild species widely.Moreover,LhMYB12 and LhMYB6 regulated anthocyanin in LA hybrids.This study revealed that the expression of R2R3-MYBs was involved in regulating various anthocyanin pigmentation patterns in different species.

关 键 词：百合 花青素苷 呈色类型 R2R3-MYBs 

分 类 号：S682.2[农业科学—观赏园艺]