乌蒙凤鸡凤头性状与Hoxc8基因表达及其生长性能的相关研究  

Correlation Between Crested Traits and Hoxc8 Gene Expression and Its Growth Performance in Wumeng Crested Chickens (Gallus gallus)

作　　者：陆静[1] 张勇[1] 骆科印 白优 张雄 李泽润 王天松 莫先艇 黄明捷 LU Jing;ZHANG Yong;LUO Ke-Yin;BAI You;ZHANG Xiong;LI Ze-Run;WANG Tian-Song;MO Xian-Ting;HUANG Ming-Jie(Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, Guizhou University, Guiyang 550025, China;College of Animal Science/Guizhou Province Key laboratory of Animal Genetics, Breeding and Reproduction, Guizhou University, Guiyang 550025, China;Agricultural Committee of Liupanshui City, Liupanshui 553000, China;Institute of Animal Husbandry and Veterinary Medicine, Guizhou Academy of Agricultural Sciences, Guiyang 550005, China)

机构地区：[1]贵州大学高原山地动物遗传育种与繁殖教育部重点实验室,贵阳550025 [2]贵州大学动物科学学院/贵州省动物遗传育种与繁殖重点实验室,贵阳550025 [3]贵州省六盘水市市农委,六盘水553000 [4]贵州省农科院畜牧兽医研究所,贵阳550005

出　　处：《农业生物技术学报》2019年第6期1062-1071,共10页Journal of Agricultural Biotechnology

基　　金：喀斯特山区特色农业发展专项(No.52020-2015-H-07);贵州省科技计划项目(黔科合支撑[2017]2533-3)

摘　　要：同源盒基因C8 (homeobox gene C8, Hoxc8)对鸡(Gallus gallus)凤头性状有着重要调节作用,且凤头性状对鸡早期生长性能有显著影响。为研究乌蒙凤鸡Hoxc8基因表达量与凤头性状及其生长性能的相关性,本研究以乌蒙凤鸡为研究对象,测定不同凤头性状乌蒙凤鸡早期生长性能及Hoxc8基因表达量。研究结果显示,Hoxc8基因在成年有凤头乌蒙凤鸡各组织中均有表达,在凤头骨中表达量最高,在睾丸中表达量较高,在肾、胸肌、腿肌、胫骨和大肠中表达量居中,在心、肝、卵巢、脾脏中表达量较低(P<0.01);在24周龄有凤头乌蒙凤鸡头皮和背皮组织中,Hoxc8基因的表达量极显著低于无凤头乌蒙凤鸡(P<0.01);Hoxc8基因在不同性别乌蒙凤鸡各组织中呈差异表达:在24周龄乌蒙凤鸡中,公鸡头皮、背皮组织中表达量极显著高于母鸡(P<0.01);在成年有凤头乌蒙凤鸡中,公鸡胫骨、腿肌组织表达量高于母鸡,母鸡心、肝、下丘脑、垂体组织表达量高于公鸡;凤头性状对乌蒙凤鸡早期生长性能有影响,随着周龄的增长,有凤头乌蒙凤鸡体重、体斜长、胸深、胸宽、胫长指标生长趋势较无凤头乌蒙凤鸡好;有凤头和无凤头乌蒙凤鸡公鸡早期生长性能优于母鸡;出壳重较大的乌蒙凤鸡早期增重较好。上述研究结果表明,乌蒙凤鸡凤头性状与Hoxc8基因头皮、背皮组织特异表达有关,有凤头乌蒙凤鸡早期生长性能较好。本研究结果对乌蒙凤鸡的保种选育有一定指导意义。Many studies have shown that homeobox C8 (Hoxc8) gene plays an important regulatory role in the crested traits of chickens (Gallus gallus), and the crested traits have a significant impact on the early growth performance of the chickens. Therefore, in order to study the correlation between the expression of Hoxc8 gene and the crested traits and growth performance of Wumen crested chickens, this study had taken the Wumeng crested chickens as research objects, measuring the early growth performance and the expression of Hoxc8 gene in different tissues. The results showed that Hoxc8 gene was expressed in all tissues of adult crested Wumeng crested chickens. It had the highest expression in crested bone;higher expression in testis;moderate expression in kidney, chest muscle, leg muscle, tibia and large intestine;and lower expression in heart, liver, ovary, hypothalamus spleen (P<0.01);Hoxc8 gene was differentially expressed in the scalp and back shin tissues of Wumeng crested chickens with 24 weeks of age, with the slight expression in crested chickens and higher expression in non-crested chickens (P<0.01). Hoxc8 gene was differentially expressed in the tissues of female and male Wumeng crested chickens: The expression of scalp and back shin tissue in cocks of 24 weeks old Wumeng crested chickens were significantly higher than that of hens (P<0.01). In adult crested Wumeng crested chickens, the expression of tibia and leg muscle tissue of cocks was higher than that of hens, and the expression of heart, liver, hypothalamus and pituitary tissue of hens were higher than that of cocks;The crested traits had an effect on the early growth performance of Wumeng crested chickens. With the increase of the age of the week, the growth trend of the body weight, body length, chest depth, chest width and tibia length of crested Wumeng crested chickens were better than that of the non-crested Wumeng crested chickens;The early growth performance of cocks with crested and non-crested Wumeng crested chickens were better than that of h

关 键 词：乌蒙凤鸡 凤头性状 体重增长 组织表达 Hoxc8 

分 类 号：S831.2[农业科学—畜牧学]