猪体细胞克隆胚胎移植技术要点分析  

Analysis on the Technical Key Points of Porcine (Sus scrofa) Somatic Cell Cloning Embryo Transfer

作　　者：吴亚林 李继良[3] 周伟良[3] 董树仁 冯保亮 刘志国[2] 牟玉莲[2] 李奎[2] WU Ya-Lin;LI Ji-Liang;ZHOU Wei-Liang;DONG Shu-Ren;FENG Bao-Liang;LIU Zhi-Guo;MU Yu-Lian;LI Kui(Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, School of Life Science and Engineering, Foshan University, Foshan 528000, China;Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China;Tianjin Ningheyuan Swinebreeding Farm, Tianjin 301504, China)

机构地区：[1]佛山科学技术学院,生命科学与工程学院,广东省分子设计与精准育种重点实验室,佛山528000 [2]中国农业科学院北京畜牧兽医研究所,北京100193 [3]天津市宁河原种猪场,天津301504

出　　处：《农业生物技术学报》2019年第6期1126-1132,共7页Journal of Agricultural Biotechnology

基　　金：转基因生物新品种培育重大专项(No.2016ZX08010004; No.2016ZX08006001);中国农业科学院基本科研业务费(No.2017ywf-zd-9);国家重点研发计划(No.2016YFF0202303-02);佛山科学技术学院高层次人才科研启动费(No.cgg07061);广东省动物分子设计与精准育种重点实验室([2019]75号)

摘　　要：猪(Sus scrofa)胚胎移植技术作为转基因、基因编辑猪制备过程中的必备技术,对制备效率有着重要影响。本文对猪体细胞克隆胚胎移植过程中的关键技术环节:受体母猪选择、受体母猪发情与移植胚胎的同期性、胚胎移植操作方法、受体猪的麻醉方法、移植时间、移植胚胎的数量、受体手术后管理等方面的技术要点、操作经验以及研究进展做讨论分析,为猪体细胞克隆胚胎移植操作提供参考。As a necessary technique in the obtaining of transgenic and gene editing pigs (Sus scrofa), embryo transfer technology has an important influence on the cloning efficiency. In this paper, the key points of pig cloned embryos transfer, such as the selection of recipient sows, the concurrent nature of receptor sow estrus and transplanted embryos, the operation method, the anesthesia method, the time of transfer, the number of transplanted embryos and the post-operative nursing, are reviewed and discussed. This paper provides reference for the operation of pig somatic cell cloning embryo transfer.

关 键 词：胚胎移植 猪 克隆效率 体细胞核移植 受体母猪 

分 类 号：S188[农业科学—农业基础科学]