梓醇对2型糖尿病大鼠心肌细胞氧化应激及心功能的影响  

Effects of catalpol on oxidative stress and cardiac function in type 2 diabetic rats

作　　者：许瑞 王丹 王研 郑秋宇[1] 史建平[1] 邹国良[3,4] Xu Rui;Wang Dan;Wang Yan;Zheng Qiuyu;Shi Jianping;Zou Guoliang(Department of Internal Cardiology,Jilin Integrated Traditional Chinese and Western Medicine Hospital,Jilin 132012,China;College of Pharmacy,Jilin Medical College,Jilin 132013,China;Department of Internal Medicine,Heilongjiang University of Traditional Chinese Medicine,Harbin 150040,China;Department of Internal Cardiology,First Affiliated Hospital of Heilongjiang University of Traditional Chinese Medicine,Harbin 150040,China)

机构地区：[1]吉林省吉林中西医结合医院心血管内科,132012 [2]吉林医药学院药学院,132013 [3]黑龙江中医药大学内科学教研室,哈尔滨150040 [4]黑龙江中医药大学附属第一医院心血管一科,哈尔滨150040

出　　处：《国际中医中药杂志》2019年第5期476-480,共5页International Journal of Traditional Chinese Medicine

基　　金：吉林省中医药科技项目(2017276);吉林省吉林市科技发展计划项目(201737111);黑龙江省博士后科研启动金(2014年).

摘　　要：目的观察梓醇对T2DM模型大鼠心肌细胞的保护作用,探讨其作用机制。方法采用随机数字表法将SD大鼠分为正常组10只和实验组90只。实验组大鼠高脂高糖饲料喂养8周后,腹腔注射STZ溶液15 mg/kg制备T2DM模型。将成模大鼠根据FPG、体重,分层随机分为模型组、二甲双胍组、低剂量组、中剂量组、高剂量组。二甲双胍组灌胃二甲双胍混悬液90 mg/kg,低、中、高剂量组分别灌胃梓醇2.5、5、10 mg/kg,正常组和模型组灌胃等体积蒸馏水。1次/d,均灌胃12周。末次给药后,测定FPG,检测心肌组织SOD、MDA水平,采用小动物超声仪检测心脏结构与心功能。结果与模型组比较,高、中、低剂量组大鼠FPG降低(P<0.05),心肌组织SOD[(197.43±8.85)U/ml、(186.54±5.89)U/ml、(175.62±7.67)U/ml比(157.75±11.29)U/ml]水平升高(P<0.05),MDA[(7.26±0.72)nmol/mg、(8.58±0.93)nmol/mg、(10.62±0.59)nmol/mg比(14.80±0.71)nmol/mg]水平降低(P<0.05);E/A值[(1.25±0.18)、(1.09±0.14)、(0.97±0.11)比(0.51±0.11)]升高(P<0.05),E/E'值[(12.33±0.73)、(13.26±1.07)、(14.73±1.23)比(20.54±1.64)]、IVRT[(21.90±2.60)ms、(22.05±2.84)ms、(24.42±2.22)ms比(27.40±2.81)ms]降低(P<0.05)。结论梓醇可抵抗心肌氧化应激,改善心脏舒张功能,对T2DM模型大鼠心肌细胞有一定的保护作用。Objective To study the protective effects of catalpol on cardiomyocytes in type 2 diabetic rats.Methods Based on the method of random number table,8-wee k-old SD rats were randomly divided into normal group (10 rats) and experimental group (90 rats).The rats in the experimental group that were fed with high-fat and high-sugar diet for 8 weeks were injected intraperitoneally with STZ (15 mg/kg),with fasting blood glucose≥16.7mmol/L as a model for type 2 diabetes.Then based on fasting blood glucose and body weight,the rats meeting the criteria of type 2 diabete mellitus were randomy divided into five group,namely,the model group,the control group,the low or medium and high doses of the catalpol group,and there were 12 rats in each group.The normal group and the model group were filled with distilled water (5 ml/kg?d,the control group were treated with metformin (90 mg/kg?d),and Low,medium and high dose group were intragastric administration of Catalpol(2.5,5,10 mg/kg?d).Then,the level of glucose,myocardial oxidative stress related protein,cardiac strcture and function change were measured after 12 weeks of intervention in rats.Results Compared with the model group,the level of fasting blood glucose in catalpol low,medium and high dose groups was significantly lower (P<0.05).The levels of SOD (197.43 ± 8.85 U/ml,186.54 ± 5.89 U/ml,175.62 ± 7.67 U/ml vs.157.75 ± 11.29 U/ml) in myocardial tissue of those groups significantly increased (P<0.05),the levels of MDA (7.26 ± 0.72 nmol/mg,8.58 ± 0.93 nmol/mg,10.62 ± 0.59 nmol/mg vs.14.80 ± 0.71 nmol/mg) in myocardial tissue of those groups significantly decreased (P<0.05),the E/A value (1.25 ± 0.18,1.09 ± 0.14,0.97 ± 0.11 vs.0.51 ± 0.11) in those groups significantly increased (P<0.05),the E/E' values (12.33 ± 0.73,13.26 ± 1.07,14.73 ± 1.23 vs.20.54 ± 1.64) and the IVRT (21.90 ± 2.60 ms,22.05 ± 2.84 ms,24.42 ± 2.22 ms vs.27.40 ± 2.81 ms) in those groups significantly decreased (P<0.05).Conclusions The catalpol can reduce oxidative stress,improve cardiac

关 键 词：梓醇 糖尿病 2型 氧化性应激 心房功能 大鼠 

分 类 号：R285.5[医药卫生—中药学]