天名精石油醚部位对小鼠巨噬细胞吞噬功能和抗体形成细胞的影响  

Effect of carpesium abrotanoides petroleum ether fraction on phagocytic function of macrophages and antibody forming cell in mice

作　　者：林力 周玉丹 徐佳琪 王子涛 肖硕 周国祥 郑江 王强[1] Lin Li;Zhou Yudan;Xu Jiaqi;Wang Zitao;Xiao Shuo;Zhou Guoxiang;Zheng Jiang;Wang Qiang(Basic Medical College of Wuhan University of Science and Technology,Wuhan 430065,China)

机构地区：[1]武汉科技大学基础医学院,430065

出　　处：《国际中医中药杂志》2019年第5期487-490,共4页International Journal of Traditional Chinese Medicine

基　　金：湖北省科技厅自然科学基金(2017CFB517);武汉科技大学大学生科技创新基金研究项目(17ZRC79).

摘　　要：目的观察天名精石油醚部位对小鼠巨噬细胞吞噬功能和抗体形成细胞的影响。方法提取天名精石油醚部位,采用随机数字表法将45只小鼠分为对照组及天名精石油醚提取物低、高剂量组,每组15只。天名精石油醚提取物低、高剂量组分别灌胃天名精石油醚提取物50、100 mg/kg,对照组灌胃等体积生理盐水,连续灌胃10 d。检测小鼠脾脏重量和生长指数,采用巨噬细胞吞噬鸡红细胞实验与溶血空斑实验检测小鼠腹腔巨噬细胞的吞噬功能与抗体形成细胞的数量。结果与对照组比较,天名精石油醚提取物低、高剂量组小鼠脾脏重量[(192.4±11.49)mg、(204.6±10.59)mg比(117.6±10.89)mg]、生长指数[(6.04±0.54)、(6.06±0.40)比(3.89±0.14)]增加(P<0.05),抗体形成细胞数[(1 216.4±94.1)、(1 548.8±86.4)比(361.0±11.7)]、巨噬细胞吞噬百分率[(58.60±2.60)%、(72.0±3.08)%比(35.49±1.64)%]、吞噬指数[(2.01±0.10)、(2.69±0.15)比(0.37±0.06)]增加(P<0.05)。结论低、高剂量的天名精石油醚部位可增强小鼠特异性和非特异性免疫功能。Objective To study the effects of carpesium abrotanoides petroleum ether fraction on phagocytic function of macrophages and antibody forming cell in mice.Methods The extract carpesium abrotanoides petroleum ether fraction,forty five mice were divided into the control group and low and high dose groups of carpesium abrotanoides petroleum ether fraction by random number table method,15 mices in each group.The low-dose and high-dose groups of carpesium abrotanoides petroleum ether fraction were given 50 mg/kg and 100 mg/kg respectively by gavage for 10 days.The spleen weight,growth index,the phagocytosis of macrophages and the number of antibody-forming cells in peritoneal macrophages of mice were detected by chicken erythrocyte phagocytosis test and hemolytic plaque test.Results Compared with the control group,the spleen weight (192.4 ± 11.49 mg,204.6 ± 10.59 mg vs.117.6 ± 10.89 mg),the growth index (6.04 ± 0.54,6.06 ± 0.40 vs.3.89 ± 0.14),antibody forming (1 216.4 ± 94.1,1 548.8 ± 86.4 vs.361.0 ± 11.7),phagocytosis percentage of macrophages (58.60%± 2.60%,72.0%± 3.08% vs.35.49%± 1.64%),and Phagocytosis index (2.01 ± 0.10,2.69 ± 0.15 vs.0.37 ± 0.06) of the groups of low doses and high doses of Carpesium abrotanoides Petroleum ether fraction significantly increased (P<0.05).Conclusions The low doses and high doses of Carpesium abrotanoides Petroleum ether fraction can enhance the mice's specific and nonspecific immune function,and protect the immune system of mice.

关 键 词：天名精 巨噬细胞 免疫功能 小鼠 

分 类 号：R285.5[医药卫生—中药学]