中药仙鹤草不同药用部位的体外药效学研究  

Pharmacodynamic Study on Different Medicinal Parts of Herba Agrimoniae in Vitro

作　　者：田露露[1] 包永睿[1,2,3] 王帅 李天娇[1,2,3] 刘金瑛 孟宪生[1,2,3] Tian Lulu;Bao Yongrui;Wang Shuai;Li Tianjiao;Liu Jinying;Meng Xiansheng(School of Pharmacy,Liaoning University of Traditional Chinese Medicine,Dalian 116600,China;Component Medicine Engineering Research Center of Liaoning Province,Dalian 116600,China;Liaoning Province Modern Chinese Medicine Research Engineerig Laboratory,Dalian 116600,China;Beijing University of Chinese Medicine ,Beijing 100029,China)

机构地区：[1]辽宁中医药大学药学院,大连116600 [2]辽宁省组分中药工程技术研究中心,大连116600 [3]辽宁省现代中药研究工程实验室,大连116600 [4]北京中医药大学,北京100029

出　　处：《世界科学技术-中医药现代化》2019年第3期408-412,共5页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：辽宁省教育厅人才项目(辽教发[2014]187号):辽宁省特聘教授,负责人:孟宪生;辽宁省教育厅高等学校创新团队项目(LT2017015):中药药效物质组学及作用机理整合研究,负责人:孟宪生;国家自然基金委面上基金项目(81874342):基于微流控细胞生物芯片技术的木蝴蝶抗肝肿瘤活性物质发现方法研究,负责人:孟宪生

摘　　要：目的:研究仙鹤草药材不同药用部位的体外抗氧化、抗肿瘤、抗凝血活性,为生药不同部位的合理应用奠定实验基础。方法:采用HPLC色谱法建立仙鹤草不同药用部位的指纹图谱;采用MTT比色法研究仙鹤草不同药用部位对人肝癌细胞株HepG2细胞、人胃癌细胞株HGC-27细胞、人结肠癌细胞株Caco-2细胞增殖作用的影响;采用2,2’-联氮-(3-乙基苯并噻唑-6-磺酸)二铵盐(ABTS)法、1,1-二苯基-2-三硝基苯肼(DPPH)法检测仙鹤草不同药用部位的抗氧化能力;采用ELISA法测定仙鹤草不同药用部位对小鼠凝血酶原(PT)指标的影响。结果:仙鹤草不同药用部位的指纹图谱之间存在明显差异,根、茎、叶、花均显示不同程度抗肿瘤、抗氧化、抗凝血药效,其中茎对HepG2、HGC-27、Caco-2三种癌细胞株的增殖抑制作用强于根、叶、花;叶、花清除DPPH、ABTS自由基的能力强于根、茎;茎、叶降低小鼠血浆凝血酶原水平的能力强于根、花。结论:仙鹤草不同药用部位的抗氧化、抗肿瘤、抗凝血药效存在差异,为仙鹤草药材以药效为导向的不同药用部位的合理使用提供实验依据,为生药不同部位的精准应用奠定实验基础。Objective: To study the antioxidant, antitumor and anticoagulant activities of different medicinal parts of Herba Agrimoniae in vitro to lay an experimental foundation for rational application of different parts of crude drugs. Methods: HPLC method was applied to study the fingerprint of different medicinal parts of Herba Agrimoniae. The proliferation inhibition rate on HepG2, HGC-27 and Caco-2 was evaluated by MTT method. ABTS method and DPPH method were used to detect the antioxidant capacity of different medicinal parts of Herba Agrimoniae. ELISA method was used to detect the effect of different medical parts of Herba Agrimoniae on PT in rats. Results: The fingerprints of different parts of Herba Agrimoniae showed significant differences. Roots, stems, leaves and flowers showed different degrees of antitumor, anti- oxidation and anti- coagulation effects. Stems had stronger inhibitory effect on the proliferation of HepG2 cells, HGC-27 cells and Caco-2 cells than roots, leaves and flowers. The ability of leaves and flowers to scavenge DPPH and ABTS free radicals was stronger than that of roots and stems, and the ability of stems and leaves to reduce plasma prothrombin level in mice was stronger than that of roots and flowers. Conclusion: The antioxidant, antitumor and anticoagulant effects of different medicinal parts of Herba Agrimoniae are different, which provides experimental basis for the rational use of different medicinal parts of Herba Agrimoniae and lays experimental foundation for the accurate application of different parts of crude drugs.

关 键 词：仙鹤草 指纹图谱 抗肿瘤 抗氧化 抗凝血 

分 类 号：R285.6[医药卫生—中药学]