针刺足三里穴对癫痫大鼠海马神经细胞凋亡的影响研究  

Effect of acupuncture at Zusanli on apoptosis of hippocampal neurons in epileptic rats

作　　者：薛小卫 刘倩 黄银兰[3] XUE Xiao-wei;LIU Qian;HUANG Yin-lan(Ningxia Chinese Medicine Hospital,Yinchuan 750021,Ningxia,China;People's Hospital of Ningxia hui Autononous Region,Yinchuan 750011,Ningxia,China;Ningxia Medical University,Yinchuan 750004,Ningxia,China)

机构地区：[1]宁夏中医医院暨中医研究院,宁夏银川750021 [2]宁夏回族自治区人民医院,宁夏银川750011 [3]宁夏医科大学,宁夏银川750004

出　　处：《时珍国医国药》2019年第4期990-993,共4页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(81560751)

摘　　要：目的通过针刺大鼠不同机能状态下的足三里穴,观察大鼠海马组织内神经细胞的早、晚期凋亡情况,以探讨足三里对大鼠海马神经细胞凋亡的影响。方法 30只SD雄性大鼠,按照随机原则,分为正常空白组、正常足三里组、模型空白组、模型足三里组共4组。其中模型组大鼠采用氯化锂-匹罗卡品(LiCl—PILO)癫痫模型。造模成功后,各组大鼠分别予以针刺干预,连续针刺10天后取材。大鼠海马组织经实验常规处理、染色,制备成单细胞悬液,后经流式细胞仪上机检测。结果与正常空白组相比,正常足三里组大鼠海马组织神经细胞早期细胞凋亡在针刺后升高,差异具有统计学意义(P<0.05),而晚期细胞凋亡的表达在针刺前后则无明显变化(P> 0.05);模型空白组与正常空白组相比,癫痫造模后,大鼠海马神经细胞早、晚期细胞凋亡表达皆升高,且差异具有统计学意义(P<0.05);模型足三里组与模型空白组相比,针刺前后大鼠海马神经细胞的早、晚期细胞凋亡表达皆无统计学意义(P> 0.05);正常足三里组与模型足三里组相比,大鼠海马神经细胞的早、晚期细胞凋亡表达皆具有统计学意义(P<0.05)。结论癫痫发生过程存在一定程度的细胞凋亡,针刺足三里穴未能起到调控癫痫发生的作用,其只对正常状态下海马神经细胞的早期凋亡具有特异性的调控作用。Objective The effects of Zusanli on the apoptosis of rat hippocampal neurons were observed by acupuncture at Zusanli Point in different functional states of rats. Methods Thirty SD male rats were divided into four groups according to the random principle, including normal blank group, normal Zusanli group, model blank group and model Zusanli group. The model group rats were treated with lithium chloride-pilocarpine(LiCl-PILO) epilepsy model. After successful modeling, rats in each group were given acupuncture intervention, and were taken after continuous acupuncture for 10 days. Rat hippocampus tissue was routinely treated and stained to prepare a single cell suspension, which was then detected by flow cytometry.Results Compared with the normal blank group, the early apoptosis of hippocampal neurons in normal Zusanli group increased after acupuncture, the difference was statistically significant(P<0.05),and the expression of late apoptosis did not change significantly before and after acupuncture(P> 0.05). Compared with the normal blank group, the expression of apoptosis in rat hippocampal neurons was increased after epileptic modeling, and the difference was statistically significant(P<0.05). Compared with the blank model group, there was no significant difference in the expression of early and late apoptosis of hippocampal neurons in the model Zusanli group before and after acupuncture(P> 0.05). Compared with the normal blank group and model Zusanli group, the expression of early and late apoptosis in rat hippocampal neurons was statistically significant(P<0.05).Conclusion There is a certain degree of apoptosis in the process of epilepsy. Acupuncture at Zusanli can not regulate the occurrence of epilepsy. It only has a specific regulation effect on the early apoptosis of hippocampal neurons in normal state.

关 键 词：足三里 癫痫 细胞凋亡 流式细胞术 

分 类 号：R245[医药卫生—针灸推拿学]