Lin28B在重度子痫前期胎盘组织中的表达及其临床意义研究  

Expression of Lin28B in the placenta of severe preeclampsia and its clinical significance

作　　者：杨云[1] 张亮[1] 李佳钋 黄岭[1] 李凡 乔宠[1] YANG Yun;ZHANG Liang;LI Jia-po;HUANG Ling;LI Fan;QIAO Chong(Key Laboratory of Maternal-Fetal Medicine of Liaoning ,Key Laboratory of Obstetrics and Gynecology of Higher Education of Liaoning Province,Shengjing Hospital of China Medical University,Shenyang 110022,China)

机构地区：[1]中国医科大学附属盛京医院辽宁省母胎医学重点实验室辽宁省高校妇产科重点实验室,辽宁沈阳110022

出　　处：《中国实用妇科与产科杂志》2019年第6期681-685,共5页Chinese Journal of Practical Gynecology and Obstetrics

基　　金：国家重点研发计划"生殖健康及重大出生缺陷防控研究"重点专项(2016YFC1000404);国家自然科学基金(81771610;81370735);盛京自由研究者基金(201706)

摘　　要：目的探讨Lin28B在重度子痫前期(SPE)胎盘组织中的表达水平及其临床意义。方法收集2017年8月至2018年8月于中国医科大学附属盛京医院住院的SPE患者40例,其中早发重度子痫前期(ESPE)组20例,晚发重度子痫前期(LSPE)组20例;选择同期妊娠晚期终止妊娠的健康孕妇40例作为对照组,其中早发对照组(N1组)20例,晚发对照组(N2组)20例。采用RT-qPCR、Western Blot、免疫组化方法检测各组胎盘组织中Lin28B mRNA及其蛋白表达水平。结果 ESPE组与N1组相比,胎盘组织中Lin28B表达强度明显降低(P<0.05);LSPE组胎盘组织中Lin28B表达强度虽低于N2组,但差异无统计学意义(P>0.05);ESPE组胎盘组织中Lin28B表达强度低于LSPE组(P<0.05)。结论 Lin28B在SPE患者胎盘组织中的表达水平明显降低,且ESPE显著低于LSPE患者,提示Lin28B可能与SPE发病和发展有关。Objective To investigate the expression and clinical significance of Lin28 B in placenta of severe preeclampsia(SPE).Methods Forty SPE patients were admitted to Shengjing Hospital of China Medical University from August 2017 to August 2018,including 20 patients with early-onset severe preeclampsia(ESPE)and 20 patients with late onset severe preeclampsia(LSPE).Another 40 healthy pregnant women who had termination of pregnancy in late pregnancy due to various reasons were selected as the control group,including 20 cases in early-onset control group(N1) and 20 cases in late-onset control group(N2). RT-qPCR analysis,Western Blot analysis and immunohistochemistry were used to detect the Lin28 B expression levels in placenta of each group.Results The expression of Lin28 B in placenta was significantly lower in ESPE group than in N1 group(P<0.05).Although its expression in LSPE group was lower than that in N2 group,the difference was not statistically significant(P>0.05).The expression of Lin28 B in placenta of ESPE group was lower than that in LSPE group(P<0.05).Conclusion The expression of Lin28 B in placenta of SPE patients is decreased,and the ESPE group was significantly lower than that in LSPE group,suggesting that Lin28 B may be associated with the pathogenesis and development of SPE.

关 键 词：Lin28B 重度子痫前期 胎盘 

分 类 号：R714.325[医药卫生—妇产科学]