解酒护肝饮解酒及对急慢酒精性肝损伤的保护作用  

Anti-Temulence Effect and Protective Mechanism of Liver-protective Oral Solution on Acute and Chronic Alcoholic Liver Injury

作　　者：丁运文 汤兴俊 陈心馨 周勤丽 罗安玲 刘小杰 丛峰松[1] Ding Yunwen;Tang Xingjun;Chen Xinxin;Zhou Qinli;Luo Anlin;Liu Xiaojie;Cong Fengsong(College of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240)

机构地区：[1]上海交通大学生命科学技术学院

出　　处：《基因组学与应用生物学》2019年第5期2276-2282,共7页Genomics and Applied Biology

摘　　要：为了评价解酒护肝饮解酒效果及其对急、慢性酒精性肝损伤保护作用机制,本研究通过建立醉酒模型,确定致醉剂量;通过醉酒睡眠实验比较解酒护肝饮解酒特性;通过测定醉酒小鼠血乙醇含量的变化,研究解酒护肝饮对乙醇代谢的影响;通过建立急慢性酒精性肝损伤模型,测定AST、ALT、SOD活性,GSH、MDA水平,HE染色切片观察肝组织形态学的变化。研究发现小鼠最佳致醉剂量为11 m L/kg;与模型组比较,解酒护肝饮高(HD)、中剂量组(MD)均可延长醉酒时间、缩短醒酒时间(p<0.05),高、中剂量组可降低酒精灌胃后2 h、3 h时间点血乙醇含量(p<0.05);与模型组比较,急慢性酒精肝损伤模型各剂量组均能显著降低血清AST、ALT活性(p<0.05),急性酒精性肝损伤模型中,各剂量组肝组织SOD、GSH水平上升(p<0.05),MDA水平下降(p<0.05),而在慢性酒精性肝损伤模型肝组织中,低剂量组(LD)的SOD、GSH及MDA水平没有统计学差异;病理切片观察可见,急慢性酒精肝损伤模型高、中、低剂量组均能显著改善肝组织因乙醇而导致的肝损伤,并且高、中剂量组效果较好。本研究表明解酒护肝饮可显著延长醉酒时间,缩短醒酒时间,降低血乙醇的含量,对酒精诱导的肝损伤有较好的保护作用。In order to evaluate the anti-temulence eff ect of Liver-protective Oral Solution and its protective mechanism on acute and chronic alcoholic liver injury. In this study, the drunk model was used to determine the best dose;drunken sleep experiments were used to compare the anti-temulence characteristics of Liver-protective Oral Solution;by measuring the change of ethanol content in temulence mice, the effects of Liver-protective Oral Solution on alcohol metabolism were studied;by establishing an acute and chronic alcoholic liver injury model, the activity of AST, ALT and SOD, the level of GSH, MDA were measured;HE staining sections were used to observe the changes of liver morphology. The study found that the best dose of mice induced dose was 11 m L/kg. Compared with the model group, it could prolong the ebriety time and shorten the sober-up time(p<0.05);the acute and chronic alcoholic liver injury model of each dose group could significantly reduce the activity of the serum AST, ALT(p<0.05);high and medium dose group could reduce blood ethanol content at 2 h, 3 h(p<0.05). In the model of acute alcoholic liver injury, the levels of SOD and GSH increased(p<0.05) and the levels of MDA decreased(p<0.05) in the liver of each group, while in the model of chronic alcoholic liver injury, the levels of SOD and GSH had no significant difference in the levels of SOD, GSH and MDA in the dose group. HE staining sections showed that liver injury caused by ethanol in the liver tissue was significantly improved in the acute, chronic alcoholic liver injury model.High and medium dose group is better. Liver-protective Oral Solution can significantly prolong the drunk time,shorten the sober-up time, reduce the blood ethanol content and have a better protective effect in alcohol-induced liver injury.

关 键 词：解酒护肝饮 酒精性肝损伤 致醉剂量 HE染色切片 

分 类 号：R285.5[医药卫生—中药学]