水稻白条纹叶突变体wsl1的遗传分析及基因精细定位  

Genetic analysis and fine mapping of white stripe leaf mutant wsl1 in rice

作　　者：莫祎 孙志忠[2] 丁佳[2] 余东[2] 孙学武[2] 盛夏冰[2] 谭炎宁[2] 袁贵龙[2] 袁定阳 段美娟[1] MO Yi;SUN Zhi-Zhong;DING Jia;YU Dong;SUN Xue-Wu;SHENG Xia-Bing;TAN Yan-Ning;YUAN Gui-Long;YUAN Ding-Yang;DUAN Mei-Juan(Hunan Agricultural University, Changsha 410128, Hunan, China;State Key Laboratory of Hybrid Rice, Hunan Hybrid Rice Research Center, Changsha 410125, Hunan, China;National South Grain and Oil Crops Collaborative Innovation Center, Changsha 410128, Hunan, China)

机构地区：[1]湖南农业大学,湖南长沙410128 [2]湖南杂交水稻研究中心杂交水稻国家重点实验室,湖南长沙410125 [3]国家南方粮油作物协同创新中心,湖南长沙410128

出　　处：《作物学报》2019年第7期1050-1058,共9页Acta Agronomica Sinica

基　　金：国家自然科学基金项目(3167166);水稻种子良种繁育关键技术研究与示范(2018YFD0100802);香港中文大学合作项目培育超高产杂交水稻组合(TK1711793);湖南省农业科学院科技创新项目(2017ZD02);湖南省自然科学青年基金项目(2017JJ3166)资助~~

摘　　要：从粳稻日本晴和籼稻R1128杂交衍生的重组自交系群体中获得一个稳定遗传的白条纹叶突变体wsl1(white stripe leaf 1),世代为F10。与亲本R1128相比,突变体wsl1表现出白条纹叶,同时叶脉呈现白化,该性状在苗期就出现并持续整个生育期;突变体的株高、每穗总粒数、剑叶长、生育期显著增加,而结实率显著下降,其他农艺性状没有显著变化。分蘖期突变体wsl1的叶绿素a、叶绿素b和胡萝卜素含量较杂交亲本R1128显著下降;透射电镜观察表明,与野生型相比,突变体的叶绿体形状异常,不规则。遗传分析表明,该突变性状由1对隐性核基因控制。精细定位后发现,目标基因WSL1位于第1染色体短臂上标记M1-54与标记M1-70之间,两者相距89.7kb。生物信息学分析表明候选区间内共有8个开放阅读框,暂未发现已报道的叶色相关基因;其中LOC_Os01g02080编码肽基脯氨酰顺反异构酶,GO(Gene Ontology)分类显示其可能与类囊体形成有关,后续将通过比较测序、qRT-PCR等分子实验来确定候选基因。A white stripe leaf mutant wsl1 was obtained from the recombinant inbred lines derived from the cross of Oryza sativa var. japonica Nipponbare and Oryza sativa L. subsp. indica R1128. The mutant wsl1 showed white striped leaves and albino veins firstly at the seedling stage and then through the whole growth period. Agronomic traits such as plant height, number of spikelets per panicle, flag leaf length and heading date were significantly increased, while the seed setting rate decreased significantly in the mutant. Compared with wild type R1128, the chlorophyll a, chlorophyll b, and carotene contents of mutant leaves obviously decreased. Microscope observation indicated there were significantly decreased normal chloroplast and a large number of abnormal chloroplasts in mutant. Genetic analysis indicated that the mutant phenotype was controlled by a single recessive nuclear gene. WSL1 was mapped on the short arm of chromosome 1, between markers M1-54 and M1-70, with physical distance of about 89.7 kb. There were eight new open reading frames in the candidate region. Among them LOC_Os01g02080 encodes a peptide-based prolyl cis-trans isomerase, GO (Gene Ontology) classification showed that it might be related to thylakoid formation.

关 键 词：水稻 白条纹叶突变体 遗传分析 基因定位 

分 类 号：S511[农业科学—作物学]