DOPA-IGF-1 Coated HA/PLGA Microspheres Promoting Proliferation and Osteoclastic Differentiation of Rabbit Bone Mesenchymal Stem Cells  

作　　者：LIU Guomin LI Yalong YANG Shuting ZHAO Yian LU Tiancheng JIA Wenyuan JI Xuan LUO Yungang 

机构地区：[1]Department of Prosthdontics,the Hospital of Stomatology,Jilin University,Changchun 130021,P.R.China [2]Department of Stomatology,the Second Hospital cf Jilin University,Changchun 130041,P.R.China [3]Life Sciences College,Jilin Agricultural University,Changchun 130118,P.R.China [4]Jilin Provincial Changbai Mountain Medicine Anti-tumor Engineering Center,the Second Hospital of Jilin University,Changchun,130041,P.R.China

出　　处：《Chemical Research in Chinese Universities》2019年第3期514-520,共7页高等学校化学研究（英文版）

摘　　要：To explore the ability of dihydroxyphenylalanine-insulin-like growth factor-1 (DOPA-IGF-1) coated hydroxyapatite/poly(lactic-co-glycolic acid)(HA/PLGA) microspheres to promote the proliferation and osteoclastic differentiation of rabbit bone mesenchymal stem cells(rBMSCs), HA/PLGA microspheres with different HA content (10%, 30%, 50%, mass fraction) were prepared by electrospinning method and HA/PLGA microspheres with 50% HA were coated with IGF-1 and DOPA-IGF-1, respectively. They were co-cultured with rBMSCs, respctively. Cell counting kit-8(CCK-8) detection, confocal laser scanning microscopy(CLSM), alkaline phosphatase(ALP) detection and osteogenesis related genes COL IA1, Runx2 and bone morphogenetic protein-2(BMP-2) detection were conducted to detect the proliferation activity, cell morphology, differentiation ability and the expression level of osteogenesis-related genes of cells cultured on all microspheres groups. The results showed that rBMSCs proliferation increased in an HA content dependent manner, and cells proliferated more in the IGF-1 coated and DOPA-IGF-1 coated groups, in particular in DOPA-IGF-1 coated group, and the differences were more remarkable over time (P<0.05). HA/PLGA microspheres promoted the proliferation and osteogenic differentiation of rBMSCs, and DOPA-IGF-1 coating enhanced the proliferation and osteogenic differentiation of rBMSCs.

关 键 词：Biomaterial ELECTROSPINNING BIOCOMPATIBILITY HYDROPHILIC polymer 

分 类 号：O6[理学—化学]