HPLC法同时测定湘产枳壳中5种成分含量的研究  

Simultaneous content determination of five constituents in Fructus Aurantii produced in Hunan,China by high-performance liquid chromatography

作　　者：刘姗姗 蒋波[1,2] 石雨荷 王丽君 刘瑞连[3] 严建业 罗堃[1] 张智敏[1] 童巧珍[1,4] LIU Shanshan;JIANG Bo;SHI Yuhe;WANG Lijun;LIU Ruilian;YAN Jianye;LUO Kun;ZHANG Zhimin;TONG Qiaozhen(Hunan University of Chinese Medicine,Changsha 410208,Hunan,China;Hunan Center for Drug Evaluation,Certification and ADR Monitoring,Changsha 410013,Hunan,China;Hunan Academy of Chinese Medicine,Changsha 410013,Hunan,China;Yueyang Municipal Hospital of Traditional Chinese Medicine,Yueyang 410400,Hunan,China)

机构地区：[1]湖南中医药大学,湖南长沙410208 [2]湖南省药品审评认证与不良反应监测中心,湖南长沙410013 [3]湖南省中医药研究院中药研究所,湖南长沙410013 [4]湖南省岳阳市中医医院,湖南岳阳410400

出　　处：《湖南中医杂志》2019年第5期143-146,共4页Hunan Journal of Traditional Chinese Medicine

基　　金：湘产大宗药材品质评价湖南省重点实验室基金项目(编号:20160211);湖南省教育厅科研项目(编号:17C1214;16C1223);湖南中医药大学一层次学科中药学资助项目(编号:湘教通[2011]76号)

摘　　要：目的:建立高效液相色谱(HPLC)法同时测定湘产枳壳中芸香柚皮苷、柚皮苷、橙皮苷、新橙皮苷和橙皮内酯的含量。方法:采用Venusil XBP C18(4. 6mm×250mm,5μm)色谱柱,以乙腈-0. 1%磷酸水为流动相(80∶20),流速1. 0ml/min,柱温35℃,变波长检测。结果:芸香柚皮苷、柚皮苷、橙皮苷、新橙皮苷、橙皮内酯分别在100～2000ng、420～8400ng、32. 12～642. 4ng、260～5200ng、42. 75～855ng内呈良好线性关系,平均回收率分别为98. 7%、101. 0%、100. 7%、100. 6%、98. 6%,且精密度、重复性、稳定性良好;各批次样品中芸香柚皮苷、柚皮苷、橙皮苷、新橙皮苷、橙皮内酯的含量范围分别为0. 48%～0. 84%、4. 44%～8. 50%、0. 19%～0. 95%、3. 03%～10. 19%、0. 16%～0. 30%;聚类分析分为3类,即11号样品为一类,2号与10号样品为一类,其余样品为一类;橙皮苷含量在各批次样本中波动较大。结论:本研究建立了湘产枳壳中5种成分的HPLC含量测定方法,该方法简便,准确度高,可为湘产枳壳质量控制提供科学依据。Objective:To establish the high-performance liquid chromatography(HPLC)method for sim-ultaneous content determination of narirutin,naringin,hesperidin,neohesperidin,and meranzin in Fructus Aurantii produced in Hunan,China.Methods:HPLC was performed on a Venusil XBP C 18 column(4.6mm×250mm,5μm)with a mobile phase of acetonitrile-0.1%phosphoric acid(80∶20)at a flow rate of 1.0ml/min and a column temperature of 35℃,with multiple wavelengths.Results:Narirutin,naringin,hesperidin,neohesperidin,and meran-zin showed a good linear relationship within the ranges of 100~2000ng,420~8400ng,32.12~642.4ng,260~5200ng,and 42.75~855ng,respectively(average recovery rate=98.7%,101.0%,100.7%,100.6%,and 98.6%),with good precision,reproducibility,and stability.The ranges of the content of narirutin,naringin,hesperi-din,neohesperidin,and meranzin were 0.48%~0.84%,4.44%~8.50%,0.19%~0.95%,3.03%~10.19%,and 0.16%~0.30%,respectively.The samples were classified into three categories based on the cluster analysis,with sample 11 in one category,samples 2 and 10 in one category,and the other samples in one category.The content of hesperidin fluctuated across samples.Conclusion:This study establishes the HPLC method for simultaneous content determination of five constituents in Fructus Aurantii produced in Hunan.This method is simple,convenient,and high-ly accurate and can provide a scientific basis for the quality control of Fructus Aurantii produced in Hunan.

关 键 词：湘产枳壳 HPLC法 含量测定 

分 类 号：R284.1[医药卫生—中药学]