山东省淄博市淄川区淡色库蚊kdr等位基因突变研究  

Study on kdr allele mutation of Culex pipiens pallens in Zichuan District, Zibo City, Shandong Province

作　　者：张伟 方圆[1] 张仪[1] 施文琦[1] 李元元 岳志远 ZHANG Wei;FANG Yuan;ZHANG Yi;SHI Wen-Qi;LI Yuan-Yuan;YUE Zhi-Yuan(National Institute of Parasitic Diseases,Chinese Center for Disease Control and Prevention,WHO Collaborating Center for Trop-ical Diseases,Key Laboratory of Parasite and Vector Biology,National Health Commission,Shanghai 200025,China;Zichuan District Center for Disease Control and Prevention,Zibo City,Shandong Province,China)

机构地区：[1]中国疾病预防控制中心寄生虫病预防控制所、WHO热带病合作中心、国家卫生健康委员会寄生虫病原与媒介生物学重点实验室,上海200025 [2]山东省淄博市淄川区疾病预防控制中心

出　　处：《中国血吸虫病防治杂志》2019年第2期134-138,共5页Chinese Journal of Schistosomiasis Control

基　　金：国家科技基础资源调查专项(2017FY101203);第四轮上海市加强公共卫生体系建设三年行动计划(GWIV-29)

摘　　要：目的了解山东省淄博市淄川区淡色库蚊(Culex pipiens pallens)季节消长趋势及蚊虫击倒抗性(Knockdown resistance,kdr)相关钠通道基因多态性分布特征。方法于2017-2018年蚊媒活动高峰期,使用诱蚊灯法对山东省淄博市淄川区进行蚊虫种群和密度调查,提取淡色库蚊DNA,通过PCR检测kdr等位基因突变类型和频率。结果在山东省淄博市淄川区共捕获830只蚊虫,包括淡色库蚊、骚扰阿蚊、白纹伊蚊、刺扰伊蚊、中华按蚊和三带喙库蚊6种,其中淡色库蚊占83.13%,密度为12.32只(/台·夜);淡色库蚊全年密度监测曲线呈双峰趋势,高峰出现在6月和9月。在kdr基因1 014 位点共检测发现5 种kdr 等位基因类型,包括TTA(75.71%)、TTT(10.00%)、CTA(5.71%)、TCA(4.29%)、TTC(4.29%),同时发现2种核苷酸非同义突变,即亮氨酸(L1014)突变为苯丙氨酸(L1014F)、丝氨酸(L1014S)。罗村镇、太河镇两地淡色库蚊kdr基因突变频率分别为10.53%、40.63%,差异具有统计学意义(χ^2 = 8.559,P = 0.003)。结论淡色库蚊为山东省淄博市淄川区优势蚊种。本研究首次在淡色库蚊中检测发现CTA、TTC两种kdr 等位基因突变,kdr 基因型呈多态性。Objective To study the seasonal dynamics of Culex pipiens pallens and the distribution of knockdown resistance (kdr) gene related sodium channel gene polymorphism in Zichuan District,Zibo City,Shandong Province.Methods Cx.pipiens pallens mosquitoes were collected in Zichuan District during the peak period of mosquito vector activity from 2017 to 2018.The DNA from Cx.pipiens pallens was extracted,and the genotypes and frequencies of kdr allele mutation were detected by polymerase chain reaction.Results Totally 830 mosquitoes belonging to six species,including Cx.pipiens pallens,Armigeres subalbatus,Aedes albopictus,Ae.vexans,Anopheles sinensis,and Cx.tritaeniorhchus were collected in this study.The number of Cx.pip- iens pallens accounted for 83.13% in total,with the density of 12.32 per lamp per night.The annual density monitoring curve of Cx.pipiens pallens showed a bimodal trend,and the peaks were observed in June and September respectively.In this study,five kdr alleles were detected at the 1 014 locus of kdr gene,with TTA (75.71%),TTT (10.00%),CTA (5.71%),TCA (4.29%),and TTC (4.29%).Two nonsynonymous nucleotide mutations were detected at site 1 014 of kdr gene,namely leucine (L1014) mutated to phenylalanine (L1014F) and serine (L1014S).The kdr gene mutation frequency (%) of Cx.pipiens pallens in Luochun Town and Taihe Town was 10.53% and 40.63%,respectively,and the difference was statistically significant (χ^2 = 8.559,P = 0.003).Con- clusions Cx.pipiens pallens is the dominant mosquito species in Zichuan District.In addition,two novel mutations,CTA and TTC,are identified in the voltage-dependent sodium channel gene of Cx.pipiens pallens.The kdr genotype of Cx.pipiens pallens in Zichuan area was polymorphic.

关 键 词：淡色库蚊 季节消长 种群密度 击倒抗性 基因型多态性 

分 类 号：R384.1[医药卫生—医学寄生虫学]