软骨寡聚基质蛋白在人升主动脉瘤中的表达及意义  

Expression and role of cartilage oligomeric matrix protein in human ascending aortic aneurysm

作　　者：秦卫[1] 曹一得 李良鹏[1] 黄福华[1] 陈鑫[1] Qin Wei;Cao Yide;Li Liangpeng;Huang Fuhua;Chen Xin(Department of Thoracic and Cardiovascular Surgery,Nanjing Medical University,Nanjing First Hospital,Nanjing 210006,China;Nanjing Medical University,Nanjing 211166,China)

机构地区：[1]南京医科大学附属南京医院南京市第一医院心胸血管外科,210006 [2]南京医科大学,211166

出　　处：《中华实验外科杂志》2019年第6期1001-1003,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金青年项目(81600225);江苏省自然科学基金青年项目(BK20160132);江苏省临床医学科技专项(BE2017610).

摘　　要：目的探讨软骨寡聚基质蛋白(COMP)在人升主动脉瘤中的表达及意义。方法收集2015年1月至2016年1月南京市第一医院术中切除的人升主动脉瘤标本作为主动脉瘤组[24例,年龄(62.70±7.56)岁,其中男22例,女2例],将心脏移植手术中获取的供心升主动脉作为非主动脉瘤组[18例,年龄(51.50±11.23)岁,其中男14例,女4例]。采用苏木精-伊红(HE)染色和Masson染色观察两组标本的组织学变化。运用蛋白质印迹法(Western blot)检测血管平滑肌细胞(VSMCs)表型蛋白平滑肌22α蛋白(SM22α)、α-肌动蛋白(SMα-actin)及骨桥蛋白(OPN)的表达水平。同时,运用Westernblot和免疫组织化学(IHC)检测两组标本中COMP的表达。应用SPSS13.0统计软件分析。符合正态分布的计量资料以均值±标准差(Mean±SD)表示,组间比较采用t检验。结果HE染色和Masson染色显示,非主动脉瘤组中血管平滑肌细胞层状排列、结构整齐、染色均匀、胞核完整、胶原沉积少;而主动脉瘤组中血管平滑肌细胞丧失了原来的梭形形态和典型整齐的层状结构,弹力蛋白断裂,胶原沉积非常明显。与非主动脉瘤比较,主动脉瘤组织中VSMCs收缩表型蛋白SM22α、SMα-actin的表达降低(1.738±0.112比1.426±0.103,t=7.527,P<0.05;1.196±0.218比0.481±0.236,t=14.640,P<0.05),差异有统计学意义,而合成表型蛋白OPN的表达明显上升(0.773±0.312比1.457±0.419,t=8.644,P<0.05)。Westernblot和IHC结果均显示,COMP的表达在主动脉瘤组中显著降低(0.395±0.109比0.128±0.085,t=9.207,P<0.05),差异有统计学意义。结论COMP在人升主动脉瘤中表达减低,其表达减低可能导致了升主动脉瘤中VSMCs的表型变化,最终促进动脉瘤的发展。Objective To study the expression and role of cartilage oligomeric matrix protein (COMP) in human ascending aortic aneurysm (AAA). Methods From January 2015 to January 2016, the ascending aorta specimens removed from AAA patients were collected as AA group [24 patients, mean age (62.70±7.56) years, 22 males and 2 females];and the ascending aorta specimens removed from donors in heart transplantations were collected as no-AA group [18 patients, mean age (51.50±11.23) years, 14 males and 4 females]. Histological changes of the two groups were observed by hematoxylin and eosin (HE) and Masson. Comparisons of the levels of SMα-actin, SM22α, OPN in the two samples were made by Western blotting. Meanwhile, a comparison of the expression of COMP in the two groups was made by both Western blotting and immunohistochemistry (IHC). Results HE and Masson showed well-structured, well-colored, clear nucleus of vascular smooth muscle cells (VSMCs), and less collagen deposition in the no-AA group. Conversely, in AA-group, VSMCs lost their original fusiform and typical orderly structures, elastic fibers fractured, collagen deposited obviously. Compared with no-AA group, the expressions of SMα-actin, SM22α, decreased significantly (1.738±0.112 vs. 1.426±0.103, t=7.527, P<0.05;1.196±0.218 vs. 0.481±0.236, t=14.640, P<0.05), while the expression of OPN increased significantly in AA group (0.773±0.312 vs. 1.457±0.419, t=8.644, P<0.05). The level of COMP was much lower in the AA group than that in no-AA group (0.395±0.109 vs. 0.128±0.085, t=9.207, P<0.05). Conclusion The expression of COMP is reduced in ascending aortic aneurysm, which may lead to phenotypic switching of VSMCs in ascending aortic aneurysm. The decreased expression COMP may contribute to aortic aneurysm in human.

关 键 词：软骨寡聚基质蛋白 升主动脉瘤 血管平滑肌细胞 

分 类 号：R543.16[医药卫生—心血管疾病]