小鼠肾小管及肾小管周围毛细血管早期病理变化的研究  

Early pathological changes of renal tubules and peritubular capillaries in mice with contrast media

作　　者：邢森 周芸[2] 胡杰[1] 蔡申华 雷彤彤 王蕴畅 张永吉 董红霖[1] Xing Miao;Zhou Yun;Hu Jie;Cai Shenhua;Lei Tongtong;Wang Yunchang;Zhang Yongji;Dong Honglin(Department of Vascular Surgery,the Second Hospital of Shanxi Medical University,Taiyuan 030001,China;Department of Renal Medicine,Shanxi Provincial People’s Hospita,Taiyuan 030012,China;Department of Anesthesiology,Shanxi Medical University,Taiyuan 030001,China)

机构地区：[1]山西医科大学第二医院血管外科,太原030001 [2]山西省人民医院肾内科,太原030012 [3]山西医科大学麻醉学系,太原030001

出　　处：《中华实验外科杂志》2019年第6期1024-1026,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金(81770695);山西省自然科学基金(201701D22111209).

摘　　要：目的观察小鼠肾脏组织中肾小管周围毛细血管(PTC)及肾小管的早期病理变化。方法C57BL/6J小鼠70只,设立正常组(N组,35只),造影剂组(CM组,35只)。CM组给予碘帕醇腹腔注射(12.48gI/kg),N组予等量生理盐水,处理后10、20、30、40、50、60min、72h各随机选取5只小鼠采血、采集双肾,检测血清肌酐(Cr)含量,常规病理检查对肾小管损伤程度进行评分、血管性血友病因子(vWF)免疫组织化学半定量分析PTC损伤程度,透射电镜观察PTC及肾小管超微结构。应用SPSS21.0统计软件分析,计数资料采用均值±标准差(Mean±SD)表示,组间比较采用单因素方差分析,组内比较采用LSD法或Dunnett’sT3法。结果与N组(39.14±12.21、34.78±14.75、34.13±11.36、31.54±7.83、37.41±17.34、37.43±14.50、35.79±18.42)比,CM组10min^72h血清Cr含量(32.33±10.78、36.15±5.34、32.29±10.12、37.02±12.38、32.58±10.14、33.35±11.11、33.59±9.29)差异无统计学意义(F=0.219,P>0.05)。10minvWF在N组、CM组阳性表达量分别为0.10±0.01、0.15±0.01差异具有统计学意义(F=23.730,P<0.05)。30min两组肾小管损伤评分(N:0.84±0.20、CM:5.66±0.61)比较差异具有统计学意义(F=260.236,P<0.05)。CM组40~60minPTC损伤较前减轻(F=10.643,P<0.05;)肾小管损伤较前恢复(F=281.324,P<0.05),两者72h基本恢复正常。电镜下PTC内皮及细胞超微结构损伤先于肾小管上皮细胞,两者在一定时间内恢复正常。结论应用造影剂后,小鼠肾脏早期出现一过性病理损伤,并且PTC损伤可能是造影剂诱导肾损伤的初始环节。Objective To observe the early pathological changes of renal tubular and peritubular capillary (PTC) in renal tissues of mice after administration of contrast medium. Methods 70 C57BL/6J mice were selected, which set up the normal group N (35) and the contrast media group CM (35). CM group were injected intraperitoneally with iopamido l(12.48 gI/kg), while only the same volume of saline was infused intravenously in group N. Five mice which were randomly selected in the two groups at 10, 20, 30, 40, 50, 60 min, 72 h after drug treatment took blood and collected renal tissue. Enzyme linked immunosorbent assay (ELISA) to detct serum creatinine. To evaluate the renal tubules injury were examined by routine pathology and Semi-quantitative analysis of the degree of papillary thyroid carcinoma (PTC) injury by Immunohistochemistry of von Willebrand Factor. Ultrastructural of peritubule capillaries and renal tubules observed by transmission electron microscopy. Results Compared with group N (39.14±12.21, 34.78±14.75, 34.13±11.36, 31.54±7.83, 37.41±17.34, 37.43±14.50, 35.79±18.42) at 10 min-72 h, there is no statistical difference in serum creatinine in group CM (32.33±10.78, 36.15±5.34, 32.29±10.12, 37.02±12.38, 32.58±10.14, 33.35±11.11, 33.59±9.29), F=0.219, P>0. 05. The positive expression of vWF in 10min in group CM (0.15±0.01) was significantly higher than that in N group (0.10±0.01), F=23.730, P<0. 05. Compared with N group (0.84±0.20), the score of renal tubule injury in 30min was significantly higher in group CM (5.66±0.61), F=260.236, P<0. 05. In CM group, the degree of renal tubule were significantly alleviated (F=281.324, P<0. 05) and PTC injury recovered obviously (F=10.643, P<0. 05) at 40-60 min. Both of them restored to normal in 72 h. The damage of PTC endothelium and cell ultrastructure was prior to that of renal tubular epithelial cells with transmission electron microscope, and both returned to normal within a certain period of time. Conclusion After the application of contrast media, tr

关 键 词：造影剂 肾小管周围毛细血管损伤 肾小管损伤 血管性血友病因子 超微结构 

分 类 号：R587.102[医药卫生—内分泌]