胆酸钠原料中有机溶剂残留的研究  

Research of residual solvents in crude sodium cholate

作　　者：李效宽 黄瑞丽 闫曦月 朱津瑞 杨光忠 LI Xiaokuan;HUANG Ruili;YAN Xiyue;ZHU Jinrui;YANG Guangzhong(National Demonstration Center for Experimental Ethnopharmacology Education,South-Central University for Nationalities,Wuhan 430074,China)

机构地区：[1]中南民族大学民族药学国家级实验教学示范中心

出　　处：《中南民族大学学报（自然科学版）》2019年第2期176-178,共3页Journal of South-Central University for Nationalities：Natural Science Edition

基　　金：国家自然科学基金资助项目(31370379)

摘　　要：为建立气相色谱-氢火焰离子化检测法(GC-FID)定量测定胆酸钠原料中乙醇、乙醚溶剂残留的新方法,将胆酸钠用乙腈提取,采用HP-INNOWax气相毛细管柱分离,以氮气为载气,流速为1.5mL·min^-1,使用氢火焰离子化检测器进行检测,进样口温度为200℃,检测器温度为250℃.结果表明:胆酸钠原料中乙醇、乙醚的百分含量分别为0.7,0.9,0.6mg·g^-1和30,40,30μg·g^-1,按《中国药典》规定,均处于正常范围内.此方法简便易操作,重现性好,可适用于胆酸钠原料药中残留溶剂的研究和检测.In order to establish a new method for the quantitative determination of residual solvents(ethanol and ether)in sodium cholate by gas chromatography equipped with flame ionization detector(GC-FID),sodium cholate was extracted by acetonitrile and separated on HP-INNO Wax column by using nitrogen as the carrier gas(1.5 mL·min^-1),with the detector temperature at 250℃and the injection port temperature at 200℃.The results indicated that the contents of ethanol and ether in several crude sodium cholate samples were 0.7,0.9,0.6 mg·g^-1 and 30,40,30μg·g^-1 respectively,which was within the specified range according to“Chinese Pharmacopeia”.This method is simple,easy to operate,and reproducible,which can be applied to the study and detection of residual solvents in crude sodium cholate.

关 键 词：气相色谱法 FID检测器 胆酸钠 

分 类 号：O657.7[理学—分析化学]