HBV cccDNA的体外细胞模型和实验小鼠模型  

In vitro cell model and mouse model of HBV cccDNA

作　　者：苏瑜 朱园飞 田青右 谢幼华 邓强 SU Yu;ZHU Yuanfei;TIAN Qingyou(Key Laboratory of Medical Molecular Virology, Department of Medical Microbiology and Parasitology, School of Basic Medical Sciences, Fudan University, Shanghai 200032, China)

机构地区：[1]复旦大学基础医学院病原生物学系医学分子病毒学重点实验室

出　　处：《临床肝胆病杂志》2019年第6期1205-1211,共7页Journal of Clinical Hepatology

基　　金：国家自然科学基金项目(81672034,81871647);上海市教育委员会科研创新计划项目(2017-01-07-00-07-E00057);传染病国家科技重大专项(2018ZX10301208)

摘　　要：HBV慢性感染仍然是世界性公共健康问题。现有抗病毒药物能够有效抑制HBV复制,却无法使之完全治愈。共价闭合环状DNA(cccDNA)是HBV复制的原始模版,具有显著的稳定性,是HBV持续感染的分子基础,也是实现感染治愈的关键靶点。由于较低的细胞内拷贝数,以及缺乏灵敏可靠的检测方法,限制了对HBV cccDNA生物合成、代谢和调控等方面的相关研究。建立合适的cccDNA研究模型有助于了解这些生物学过程。综述了近年来HBV cccDNA细胞培养模型和实验小鼠模型等方面的主要进展,以期为进一步研究HBV病毒学和抗病毒药物研发提供帮助。Chronic hepatitis B virus(HBV) infection remains a major public health threat worldwide.Current antiviral drugs can effectively inhibit the replication of HBV,but they fail to achieve a complete cure.As the original template for HBV replication,covalently closed circular DNA(cccDNA) is intrinsically stable in vivo and is regarded as the molecular basis for persistent HBV infection and the key target for the cure of HBV infection.Studies on biosynthesis,metabolism,and regulation of HBV cccDNA have been limited by the low copy number of cccDNA within cells and the lack of sensitive and reliable detection methods.Establishment of appropriate research models of cccDNA helps to understand related biological processes.This article reviews the latest research advances in cell models and mouse models of HBV cccDNA,in order to facilitate future studies on HBV virology and development of antiviral drugs against HBV.

关 键 词：乙型肝炎病毒 共价闭合环状DNA 细胞培养模型 模型 动物 

分 类 号：R512.62[医药卫生—内科学]