高效液相色谱法测定唇用化妆品中对位红含量  

Determination of para red in lip cosmetics by high performance liquid chromatography

作　　者：侯广月 周莉莉 卞英芳 许士明 杜营 邹惠玲 HOU Guang-yue;ZHOU Li-li;BIAN Ying-fang;XU Shi-ming;DU Ying;ZOU Hui-ling(National Supervision and Inspection Center for Processed Food Quality(Shandong),Shandong Product Quality Inspection Research Institute, Jinan, Shandong 250102, China)

机构地区：[1]山东省产品质量检验研究院国家加工食品质量监督检验中心(山东)

出　　处：《日用化学工业》2019年第6期415-418,共4页China Surfactant Detergent & Cosmetics

基　　金：国家标准委员会计划项目资助(20160677-T-607);国家质量监督检验检疫总局科技计划项目资助(2017QK077);山东省标准化项目资助(2017-1-052)

摘　　要：建立了一种快速检测唇用化妆品中对位红含量的高效液相色谱分析方法。样品经三氯甲烷分散后,用乙腈进行超声提取,过滤后,采用Waters SunFire C18色谱柱(4.6 mm×250 mm,5 μm)分离,以乙腈-水溶液(体积比为85∶15)为流动相进行等度洗脱,流速为1.0 mL/min,柱温30 ℃,二极管阵列检测器检测,外标法定量。结果表明,对位红在0.1~10 mg/L质量浓度范围内线性关系良好,相关系数为0.999 9,方法检出限为0.20 μg/g,定量限为0.60 μg/g, 3组加标浓度的平均回收率为94.3%~103.8%。方法操作简便、快速准确、重现性好,适用于唇用化妆品中对位红的测定。A high performance liquid chromatography(HPLC)method for the rapid determination of para red in lip cosmetics was established. The sample was dispersed by trichloromethane, ultrasonically extracted with acetonitrile, filtered, separated by a Waters SunFire C18 column(4.6 mm×250 mm, 5 μm), and eluted equivalently with acetonitrile-water solution(volume ratio 85:15)as mobile phase. The elution was performed at a flow rate of 1.0 mL/min and the column temperature was set at 30 ℃. The analysis was done using photo-diode array detector. External standard approach was used for quantification. The results indicate that para red has a good linear relationship in the range of 0.1-10 mg/L and the correlation coefficient is 0.999 9. The limit of detection(LOD)is 0.20 μg/g, while the limit of quantification(LOQ)is within 0.60 μg/g. The average recoveries were in the range of 94.3%-103.8% at three spiked concentrations. The method is simple, rapid, accurate and reproducible, and is suitable for the determination of para red in lip cosmetics.

关 键 词：化妆品 高效液相色谱法 对位红 

分 类 号：TQ658[化学工程—精细化工]