纯水中检测汞离子与银离子的菲啰啉类荧光探针  

A Phenanthroline Based Fluorescence Probe for Identification of Hg2+ and Ag+ in Pure Aqueous Medium

作　　者：廖贤 袁剑英 牟兰[1] 曾晞[1] LIAO Xian;YUAN Jian-ying;MU Lan;ZENG Xi(Key Laboratory of Macrocyclic and Supramolecular Chemistry of Guizhou Province,College of Chemistry and Chemical Engineering,Guizhou University,Guiyang 550025,China)

机构地区：[1]贵州省大环化学及超分子化学重点实验室贵州大学化学与化工学院

出　　处：《分析测试学报》2019年第6期706-711,共6页Journal of Instrumental Analysis

基　　金：国家自然科学基金项目(21165006);教育部“春晖计划”项目(Z2016008)

摘　　要：合成了菲啰啉类化合物探针1,并通过核磁共振波谱(NMR)、质谱(MS)、红外光谱(IR)对其结构进行了表征。利用荧光发射光谱考察了探针1对Hg2+及Ag+的识别性能。结果表明,探针1在纯水介质中以荧光猝灭的方式识别Hg^2+和Ag^+,其结合比分别为1∶1和2∶1,检测的线性范围分别为9.0×10^-7~1.1×10^-5和8.0×10^-7~7.0×10^-6mol/L,相关系数(r2)均大于0.99,检出限均低至10-8mol/L。根据探针1识别前后荧光强度的剧烈变化,建立了裸眼检测Hg2+和Ag+的可视化分析方法。通过等温量热滴定与1HNMR滴定考察了识别过程的热力学参数和作用机理。该研究对生物体及环境领域中Hg2+和Ag+的实时监测具有潜在的应用价值。A phenanthroline based fluorescent probe 1 was synthesized.The structure of the probe was characterized by nuclear magnetic resonance spectroscopy (NMR ),masss pectrometry (MS ) and infrared spectroscopy (IR ),and the recognition performance of the probe toward Hg^2+ and Ag^+ was investigated by fluorescence emission spectroscopy.Results indicated that Hg^2+ and Ag^+ in pure water medium were recognized by the probe via fluorescence quenching with the ratios of 1 ∶ 1 and 2 ∶ 1 ,respectively.There were linear relationships for Hg^2+ and Ag^+ in the concentration ranges of 9.0×10^-7 -1.1×10^-5 mol/L and 8.0×10^-7 -7.0×10^-6 mol/L,respectively,with their correlation coefficients ( r^2 ) larger than 0.99.The limits of detection were both as low as 10 -8 mol/L.A visual analysis method was established for the detection of trace Hg^2+ and Ag^+ using the probe based on the obvious changes of fluorescence intensity.The thermodynamic parameters and recognition mechanism were evaluated using isothermal titration calorimetry (ITC ) and 1H NMR titration.This method has a potential application value for the real-time monitoring of Hg 2+ and Ag + in the biological and environmental fields.

关 键 词：纯水介质 菲啰啉衍生物 荧光探针 汞离子 银离子 

分 类 号：O657.3[理学—分析化学]