CBM的结构、应用及与多糖的相互作用研究进展  

Current Advances in the Structure, Applications of CBM, and the Interactions between CBM and Polysaccharides

作　　者：张雨 王晓燕 张鑫宇 王沛沛 田静 宋君龙[1,3] ZHANG Yu;WANG Xiao-yan;ZHANG Xin-yu;WANG Pei-pei;TIAN Jing;SONG Jun-long(Jiangsu Provincial Key Lab of Pulp and Paper Science and Technology, Nanjing Forestry University, Nanjing 210037, China;Dinano Tech Co., Ltd. Nanjing Branch, Nanjing 210046, China;Jiangsu Co-Innovation Center for Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University,Nanjing 210037, China)

机构地区：[1]南京林业大学江苏省制浆造纸科学与技术重点实验室,江苏南京210037 [2]鼎纳科技有限公司南京分公司,江苏南京210046 [3]南京林业大学江苏省林业资源高效加工利用协同创新中心,江苏南京210037

出　　处：《纤维素科学与技术》2019年第2期66-73,84,共9页Journal of Cellulose Science and Technology

基　　金：国家自然科学基金(31730106、31770623);江苏省高校自然基金重大项目(17KJA530005);六大人才高峰项目(2015-JNHB-013);江苏高校优势学科建设工程资助项目(PAPD)

摘　　要：碳水化合物结合模块(CBM)是碳水化合物活性酶的重要组成部分,其功能是识别并结合到多糖底物上以增加催化模块在底物上的浓度并提高活性。不同家族的CBM具有不同的结构和结合特异性,与底物的相互作用也存在差异。CBM与多糖表面的特异性吸附决定了蛋白质-多糖复合材料的化学性能和机械性能。从CBM的结构与分类、功能与应用、CBM与多糖的相互作用与检测方法等方面对CBM近年的研究进行了综述,特别是对其作为独立功能单元运用到纤维改性,生物纯化和生物修复方面的应用进行了总结。Carbohydrate-binding modules (CBM) can recognize and bind to the surface of cellulose. The different family’s CBMs have different structures and binding specificities, leading to diverse interaction with cellulose substrates. Meanwhile, the specific adsorption of CBM onto the cellulose surface determines the chemical and mechanical properties of protein-cellulose composites. In this review, the current advances on the interactions between CBMs and cellulose were surveyed, from the aspects of structures, functions and applications of CBM, especially on the applications in fiber modification, protein purification and bioremediation, as well as the monitoring methods between CBM and cellulose.

关 键 词：碳水化合物结合模块 多糖 相互作用 吸附 石英晶体微天平 

分 类 号：Q819[生物学—生物工程]