流动注射-化学发光增强法测定L-半胱氨酸  

Flow Injection Analysis of L-Cysteine with Enhanced Chemiluminescence Detection

作　　者：赵丹华 刘志浩 余文栋 周燕玲 邱美璇 朱思丽 HAO Danhua;LIU Zhihao;YU Wendong;ZHOU Yanling;QIU Meixuan;ZHU Sili(Department of Chemistry,Guangdong University of Education,Guangzhou,Guangdong,510303,P.R.China)

机构地区：[1]广东第二师范学院化学系

出　　处：《广东第二师范学院学报》2019年第3期68-72,共5页Journal of Guangdong University of Education

基　　金：广东省普通高校先进材料与节能减排工程技术开发中心项目“新型环保多孔吸附微球的可控制备及应用”(2016GCZX007);广东第二师范学院大学生创新实验项目“改性膨胀石墨烯吸附剂的制备及应用”(201814278097)

摘　　要：在碱性条件下,L-半胱氨酸对鲁米诺-过氧化氢反应所产生的化学发光有明显的增强作用.对L-半胱氨酸影响该反应体系化学发光强度的影响因素(鲁米诺、过氧化氢、pH值、泵速)进行实验研究,建立一种流动注射-化学发光增强法测定L-半胱氨酸的新方法.研究表明,在最佳实验条件下,该方法测定L-半胱氨酸的线性范围为2.0×10^-6~1.0×10^-5g/mL(r=0.9975),检出限为3.5×10^-7g/mL,对6.0×10^-6g/mL的L-半胱氨酸进行11次平行测定,RSD为3.6%.In alkaline medium,L-Cysteine has a significant enhancement effect on the chemiluminescence produced by the reaction of luminol and hydrogen peroxide.The influence factors of L-Cysteine on the chemiluminescence intensity of the reaction system (Luminol,hydrogen peroxide,pH,pump speed) were studied in detail.The analytical approach based on the system was established under the optimum experimental conditions.The CL intensity was linear with L-Cysteine concentration in the range of 2.0×10^-6 ~1.0×10^-5 g/mL ( r =0.997 5),and the detection limit was 3.5×10^-7 g/mL.The relative standard deviation of 11 determinations was 3.6% for 6.0×10^-6 g/mL L-Cysteine.

关 键 词：L-半胱氨酸 鲁米诺-过氧化氢 流动注射 化学发光 

分 类 号：O657.39[理学—分析化学]