低氟铝长期与高氟铝短期暴露后大鼠骨生长的比较研究  

Comparison of the bone growth in rats after short-term exposure to high fluoride and aluminum and long-term exposure to low fluoride and aluminum

作　　者：黄连芳 陈艳 HUANG Lianfang;CHEN Yan(Guangdong Key Laboratory for Research and Development of Natural Drugs,Guangdong Medical University,Zhanjiang 524023,China)

机构地区：[1]广东医科大学广东天然药物研究与开发重点实验室

出　　处：《中国实验动物学报》2019年第3期359-365,共7页Acta Laboratorium Animalis Scientia Sinica

基　　金：广东省科技计划项目(2015A030302077)~~

摘　　要：目的观察不同剂量氟铝联合摄入时间长短对大鼠纵向骨生长及骨代谢的影响。方法48只8周龄体重170~190g清洁级SD大鼠,随机分成正常对照,低氟铝和高氟铝组,且分别设45d和90d组。进行胫骨近端生长板和干骺端松质骨的骨形态计量学分析。结果与正常组比较,高氟铝组生长板增厚,45d组软骨细胞层次清楚,排列整齐,形态无异常,而90d组软骨细胞拥挤,潴留;低氟铝(45d和90d)组干骺端次级小梁骨矿化周长、骨形成率、成骨细胞周长都增加,且骨吸收周长在90d组增加;上述骨代谢指标在高氟铝45d组均增加,90d组均降低。结论高氟铝短期暴露刺激软骨生长,长期抑制纵向骨生长。低氟铝短期暴露只增加次级小梁骨形成,低氟铝长期与高氟铝短期暴露均可刺激小梁骨形成,增加骨吸收,高氟铝长期抑制骨形成和吸收。Objective To investigate the effects of different exposure periods of intake of highor low-dose fluoride combined with aluminum exposure on longitudinal bone growth and bone metabolism in rats.Methods Forty-eight 2-month-old Sprague-Dawley rats were randomly divided into a vehicle control group and two groups exposed to fluoride combined with aluminum (0.1 mg/kg) as follows: low-dose fluoride (5 mg/kg)+ aluminum,and high-dose fluoride (30 mg/kg)+ aluminum.In these two groups,the durations of intervention were 45 and 90 days,respectively.At the end of the experiment,all proximal tibias were harvested and processed for bone histomorphometric analysis of both the epiphyseal growth plate and epiphyseal trabeculae.Results Compared with the findings in the control group,increased growth plate thickness accompanied by a neat arrangement and normal morphology of chondrocytes was observed at 45 days in both treatment groups.In addition,hypertrophy and retention of chondrocytes was observed at 90 days in the high-fluoride + aluminum group.Percent mineralized surface,bone formation rate,and osteoblast circumference were increased in the low-dosage fluoride + aluminum group at both 45 and 90 days,while bone resorption perimeter was increased in secondary trabeculae at day 90 compared with the age-matched controls.Bone formation and bone resorption parameters mentioned above were all elevated in the high-fluoride + aluminum group at day 45,but were reduced with bone volume loss in the same group at day 90.Conclusions Short-term exposure to high-fluoride + aluminum stimulates the growth of plate cartilage,while long-term exposure leads to cartilage osteogenesis.Short-term exposure to low-fluoride + aluminum only increased secondary bone formation,whereas both long-term exposure to low-fluoride/aluminum and short-term exposure to high-fluoride/aluminum can stimulate bone formation along with bone resorption in secondary trabeculae;in contrast,long-term high-fluoride + aluminum exposure has an inhibitory effect on the longitudin

关 键 词：氟 铝 暴露时间 骨代谢 骨形态计量学 胫骨 大鼠 

分 类 号：Q95-33[生物学—动物学]