姜黄素对沙漠干热环境中暑大鼠血液和组织中性粒细胞的影响  

Effects of curcumin on the neutrophils in blood and tissues of heatstroke rats in a dry-heat desert environment

作　　者：张东辉[1] 刘江伟[1] 康燕 许琴[1] 李建瑛[1] 李佳佳[1] 是文辉[1] 董翔[1] 马娜[1] 宋来阳[1] ZHANG Donghui;LIU Jiangwei;KANG Yan;XU Qin;LI Jianying;LI Jiajia;SHI Wenhui;DONG Xiang;MA Na;SONG Laiyang(Xinjiang Key Laboratory of Special Environmental Medicine,General Hospital of Xinjiang Military Command,Urumqi 830000,China;The 69240 Hospital of Chinese PLA,Urumqi 830000)

机构地区：[1]新疆军区总医院新疆特殊环境医学重点实验室,乌鲁木齐830000 [2]解放军69240部队医院,乌鲁木齐830000

出　　处：《中国比较医学杂志》2019年第6期80-85,共6页Chinese Journal of Comparative Medicine

基　　金：军队临床高新技术重大项目(2010gxjs016);新疆自治区自然科学基金面上项目(2015211C231)

摘　　要：目的 探讨姜黄素预处理对沙漠干热环境中暑大鼠血液和组织中性粒细胞数量变化的影响。方法选取SPF级雄性SD大鼠160只,依据姜黄素灌胃剂量随机分为4组,各组连续等体积灌胃1周后,将大鼠置于西北特殊环境人工实验舱,设定沙漠干热环境气候模式,分别在0 min、50 min、100 min、150 min时间点,各组随机取10只出舱麻醉,测定血液中性粒细胞数;于150 min时间点取各组大鼠回肠、肝、肾、肺组织,HE染色、镜下观察,记录浸润的中性粒细胞数及组织病变。结果 各组的血液中性粒细胞在0 min至50 min时呈上升趋势;各组从50 min至150 min血液中性粒细胞呈下降趋势。第150 min回肠、肝、肾、肺的中性粒浸润数随着姜黄素预处理浓度的增加,均呈下降趋势,中、高浓度组中性粒细胞数明显低于低浓度组和盐水组(P<0.01)。150 min时达到热射病状态时,Pearson相关性分析发现血液中性粒细胞数与组织中性粒浸润数呈明显负相关。结论 在干热环境持续暴露下,大鼠血液中性粒细胞数呈现先升高后逐渐降低趋势;伴随着组织损伤的加重,组织浸润的中性粒细胞数呈上升趋势,血液中性粒细胞的下降与组织中性粒浸润细胞的增加呈密切相关。姜黄素可能通过抑制中暑大鼠血液中性粒细胞的下降,减少组织中性粒细胞浸润来减轻组织损伤。Objective To investigate the effects of curcumin pretreatment on the numbers of neutrophils in blood and tissues of heatstroke rats in a dry-heat desert environment.Methods One-hundred-sixty male Sprague-Dawley rats were randomly divided into four groups(n=40 per group).All groups were given an isometric gavage for 7 days.On the eighth day,all groups were transferred to a Simulated Climate Cabin,which was set to mimic the special environment of Northwest China.At 0,50,100,and 150 min,ten rats per group were removed from the cabin and anesthetized to collect blood from the inferior vena cava to determine the neutrophil number.At 150 min,the ileum,liver,kidney,and lung of the rats were harvested for pathological observation to assess the number of neutrophils and tissue alterations.Results Numbers of neutrophils in the blood of all four groups were increased from 0 to 50 min,and then decreased from 50 to 150 min.At 150 min,numbers of neutrophils infiltrating the ileum,liver,kidney,and lung tissues were decreased with the increasing curcumin dose pretreatment.Moreover,significantly fewer neutrophils were observed in the medium-and high-dose curcumin pretreatment groups compared with the saline and low-dose curcumin pretreatment groups(P<0.01).At 150 min,the rats reached a severe heatstroke status.Pearson correlation analysis indicated that the number of neutrophils in blood was negatively and significantly correlated with numbers of neutrophils in the ileum,liver,kidney and lung tissues.Conclusions With continued exposure to the desert dry-heat environment,blood neutrophils in rats are first elevated and then gradually decreased.In addition,numbers of infiltrating neutrophils in organ tissues are increased with the aggravation of heatstroke,and the decrease of blood neutrophils is intimately correlated to the increase of infiltrating neutrophils in organ tissues.By inhibiting the decrease of blood neutrophils and accompanying increase of infiltrating neutrophils in organ tissues,curcumin may alleviate organ injury i

关 键 词：姜黄素 干热环境 中暑 大鼠 中性粒细胞 

分 类 号：R-33[医药卫生]